PXD023362 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry |
Description | Glycosaminoglycans (GAGs) are essential functional components of the extracellular matrix (ECM). Artificial GAGs like sulfated hyaluronan (sHA) exhibit pro-osteogenic properties and boost healing processes and hence are of high interest for supporting wound healing. Although sulfated GAGs (sGAGs) are internalized into cells, knowledge of intracellular effects and interaction partners is scarce. Here we used an affinity-purification mass spectrometry-based (AP-MS) approach to identify novel and particularly intracellular sGAG-interacting proteins from human bone marrow stromal cells (hBMSC). Overall, 478 proteins were found interacting with at least one of four distinct sGAGs. Enrichment analysis for protein localization showed that mainly intracellular and cell-associated interacting proteins were identified. Only a limited fraction of about 10 % was annotated for extracellular regions. The interactions of the 2-macroglobulin receptor-associated protein (LRPAP1), exportin-1 (XPO1), and Serine protease HTRA1 (HTRA1) were confirmed in reverse assays. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-03 |
AnnouncementXML | Submission_2021-11-03_07:14:58.388.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Henning Großkopf |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-01-04 01:48:48 | ID requested | |
⏵ 1 | 2021-11-03 07:14:59 | announced | |
Publication List
Gro, ß, kopf H, Vogel S, M, ü, ller CD, K, ö, hling S, D, ü, rig JN, M, ö, ller S, Schnabelrauch M, Rademann J, Hempel U, von Bergen M, Schubert K, Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry. Biol Chem, 402(11):1427-1440(2021) [pubmed] |
Keyword List
submitter keyword: biotin-GAG, hBMSC, LC-MS/MS, pull-down approach, SaOS2/osteoblast cell line, sulfated glycosaminoglycan derivatives |
Contact List
Martin von Bergen |
contact affiliation | Department of Molecular Systems Biology, Helmholtz Centre for Environmental Research – UFZ, Leipzig, Germany |
contact email | martin.vonbergen@ufz.de |
lab head | |
Henning Großkopf |
contact affiliation | Molecular Systems Biology Helmholtz Centre for Environmental Research GmbH - UFZ Leipzig, Germany |
contact email | henning.grosskopf@ufz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD023362 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023362
- Label: PRIDE project
- Name: Identification of intracellular glycosaminoglycan-interacting proteins by affinity purification mass spectrometry