PXD023361-1

PXD023361 is an original dataset announced via ProteomeXchange.

Title	The Cdk8 kinase module regulates Mediator-RNA polymerase II interaction
Description	We use biochemically reconstituted transcription initiation components including the Mediator core and Cdk8 kinase modules (CKM) to investigate the function of the CKM in regulating the Mediator-RNA polymerase II interaction in a highly purified system. We use cross-linking coupled to mass spectrometry to map the interaction of the CKM and core Mediator, and in vitro phosphorylation assays followed by phosphopeptide enrichment coupled to mass spectrometry to identify Cdk8 phosphorylation targets in this context and biochemically dissect their functions. Finally, we investigate the function of phosphorylation by Cdk8 on transcription in vivo. Based on that, we propose a model to integrate the phosphorylation-dependent and -independent functions of the CKM in transcription initiation.
HostingRepository	PRIDE
AnnounceDate	2021-04-28
AnnouncementXML	Submission_2021-04-28_10:27:03.750.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fanni Laura Bazso
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 01:48:22	ID requested
⏵ 1	2021-04-28 10:27:04	announced

Dataset with its publication pending

submitter keyword: RNA polymerase II, transcription, Mediator, Cdk8

Henning Urlaub
contact affiliation	Max Planck Institute for Biophysical Chemistry, Göttingen,Germany and Clinical Chemistry, University Medical Center Göttingen, Göttingen, Germany
contact email	henning.urlaub@mpibpc.mpg.de
lab head
Fanni Laura Bazso
contact affiliation	Max Planck Institute for Biophysical Chemistry
contact email	fanni-laura.bazso@mpibpc.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023361
     1. Label: PRIDE project
     2. Name: The Cdk8 kinase module regulates Mediator-RNA polymerase II interaction