PXD023353-1

PXD023353 is an original dataset announced via ProteomeXchange.

Title	20S proteasomes exported by the malaria parasite promote its growth
Description	Mature Red Blood Cells (RBCs) lack internal organelles and canonical defense mechanisms, making them both a fascinating host cell, in general, and an intriguing choice for the deadly malaria parasite Plasmodium falciparum (Pf), in particular. Pf, while growing inside its natural host, the human RBC, secretes multipurpose extracellular vesicles (EVs), yet their influence on this essential host cell remains unknown. Here we demonstrate that Pf parasites, cultured in fresh human donor blood, export within such EVs assembled and functional 20S proteasome complexes (EV-20S). The EV-20S proteasomes modulate the mechanical properties of naïve human RBCs by remodeling their cytoskeletal network. Furthermore, we identify four novel degradation targets of the exported 20S proteasome, the phosphorylated cytoskeletal proteins β-adducin, ankyrin-1, dematin and Epb4.1. Overall, our findings reveal a previously unknown 20S proteasome export mechanism employed by the human malaria parasite, which primes RBCs for parasite invasion by altering membrane stiffness, to facilitate malaria parasite growth.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_13:59:00.552.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023353
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Eugene Kapp
SpeciesList	scientific name: Plasmodium falciparum 3D7; NCBI TaxID: 36329;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-04 00:39:25	ID requested
⏵ 1	2021-03-04 13:59:00	announced
2	2021-04-06 02:18:16	announced	2021-04-06: Updated publication reference for PubMed record(s): 33608523.

Dataset with its publication pending

submitter keyword: RBCs, Malaria, EVs, proteasome

Neta Regev-Rudzki
contact affiliation	Department of Bimolecular Sciences, Weizmann Institute of Science, Rehovot 7610001, Israel
contact email	neta.regev-rudzki@weizmann.ac.il
lab head
Eugene Kapp
contact affiliation	CSL Innovation Ltd.
contact email	Eugene.Kapp@csl.com.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/02/PXD023353

PRIDE project URI

• PRIDE
  1. PXD023353
     1. Label: PRIDE project
     2. Name: 20S proteasomes exported by the malaria parasite promote its growth