PXD023346-2
PXD023346 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | N- and O-glycosylation patterns of the SARS-CoV-2 spike protein reveal a new “O- follow-N” rule |
Description | Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), the causative agent of COVID-19, has caused a worldwide pandemic since its discovery at the end of 2019. The spike (S) protein of SARS-CoV-2 is responsible for the host cell entry and the critical targets of the immune system. Consequently, S protein has been extensively used in antibody and vaccine studies. Here, we identified a total of 23 N-glycosites, including N334 in the noncanonical consensus motif N-X-C, on the full-length wild-type S protein protomer. Furthermore, 55 O-glycosylation sites were identified, and this uncovered a pattern of O-glycosylation near N-glycosites, which we named the “O-follow-N” rule, revealed by site-specific mutagenesis. The comprehensive mapping of the S protein O- and N-glycosylation landscape provides further information for studying the mechanisms underlying viral pathogenesis and immune evasion. |
HostingRepository | iProX |
AnnounceDate | 2021-03-24 |
AnnouncementXML | Submission_2023-02-20_22:58:00.995.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Shuaixin Gao |
SpeciesList | scientific name: Severe acute respiratory syndrome coronavirus 2; NCBI TaxID: 2697049; |
ModificationList | N-glycosylated residue; O-glycosyl-L-serine; O-glycosyl-L-threonine |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-01-04 00:19:13 | ID requested | |
1 | 2021-01-04 00:22:49 | announced | |
⏵ 2 | 2023-02-20 22:58:01 | announced | 2023-02-21: Update information. |
Publication List
Tian W, Li D, Zhang N, Bai G, Yuan K, Xiao H, Gao F, Chen Y, Wong CCL, Gao GF, O-glycosylation pattern of the SARS-CoV-2 spike protein reveals an "O-Follow-N" rule. Cell Res, 31(10):1123-1125(2021) [pubmed] |
Keyword List
submitter keyword: SARS-CoV-2, spike protein,O-glycosylation sites,O- follow-N |
Contact List
Catherine CL Wong | |
---|---|
contact affiliation | Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University |
contact email | catherine_wong@bjmu.edu.cn |
lab head | |
Shuaixin Gao | |
contact affiliation | Center for Precision Medicine Multi-Omics Research, Peking University Health Science Center, Peking University |
contact email | gaoshuaixin@126.com |
dataset submitter |
Full Dataset Link List
iProX dataset URI |