PXD023334-2

PXD023334 is an original dataset announced via ProteomeXchange.

Title	Comparison of unit resolution versus high-resolution accurate mass for parallel reaction monitoring
Description	Parallel reaction monitoring (PRM) is an increasingly popular alternative to selected reaction monitoring (SRM) for targeted proteomics. PRM’s strengths over SRM are that it monitors all product ions in a single spectrum, thus eliminating the need to select interference-free product ions prior to data acquisition, and that it is most frequently performed on high-resolution instruments, such as quadrupole-orbitrap and quadrupole-time of flight instruments. Here, we show that the primary advantage of PRM is the ability to monitor all transitions in parallel, and that high-resolution data are not necessary to obtain high quality quantitative data. We run the same scheduled PRM assay, measuring 432 peptides from 126 plasma proteins, multiple times on a Orbitrap Eclipse Tribrid mass spectrometer, alternating separate liquid chromatography-tandem mass spectrometry runs between the high resolution Orbitrap and the unit resolution linear ion trap for PRM. We find that both mass analyzers have similar technical precision, and that the linear ion trap’s superior sensitivity gives it better lower limits of quantitation on over 62% of peptides in the assay.
HostingRepository	PanoramaPublic
AnnounceDate	2021-08-19
AnnouncementXML	Submission_2021-08-19_08:58:20.125.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Lilian Heil
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl; Oxidation; Label:13C(6)15N(2); Label:13C(6)15N(4)
Instrument	Orbitrap Eclipse; Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-01-02 11:51:35	ID requested
1	2021-05-06 09:38:11	announced
⏵ 2	2021-08-19 08:58:20	announced	: Added PubMed Id

Heil LR, Remes PM, MacCoss MJ, Comparison of Unit Resolution Versus High-Resolution Accurate Mass for Parallel Reaction Monitoring. J Proteome Res, 20(9):4435-4442(2021) [pubmed]

submitter keyword: mass spectrometry, limit of quantitation, precision, PRM

Michael MacCoss
contact affiliation	University of Washington
contact email	maccoss@uw.edu
lab head
Lilian Heil
contact affiliation	University of Washington
contact email	lheil2@uw.edu
dataset submitter

Panorama Public dataset URI