PXD023327-1

PXD023327 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	DNA damage-induced alternative splicing of p53
Description	Samples were reduced in loading buffer and separated by SDS-PAGE for 3-4 min followed by in-gel digestion with trypsin. Digests were analyzed by LC-MS/MS using a Waters nanoACQUITY interfaced to a Thermo Fusion Lumos MS. The LC separation 90 min gradient of 5-30% MeCN in a trapping configurations with a 75 um x 25 cm analytical column (Waters HSS T3). MS used orbitrap MS1 and iontrap MS/MS (OT-IT). Raw files were converted to .mgf using Proteome Discoverer and searched against a Swissprot human database appended with p53beta sequence and containing reverse decoys, with fixed carbamidomethyl modification on Cys and variable deamidation (NQ), oxidation (M), peptide N-terminal Gln-->pyroGlu and protein N-terminal acetylation. Data was annotated at a 1% peptide and protein FDR using Scaffold.
HostingRepository	MassIVE
AnnounceDate	2023-03-28
AnnouncementXML	Submission_2023-03-28_19:31:11.195.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Matt Foster
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	Deamidated; Carbamidomethyl; Gln->pyro-Glu; Acetyl
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-30 11:27:48	ID requested
⏵ 1	2023-03-28 19:31:11	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: p53, BioID, BirA, GFP

submitter keyword: p53, BioID, BirA, GFP

Contact List

Michael Kastan, M.D., Ph.D.
contact affiliation	Duke University
contact email	michael.kastan@duke.edu
lab head
Matt Foster
contact affiliation	Duke University
contact email	mwfoster@duke.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086651/