PXD023315 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic Analysis of Xinjiang Black Bee's Royal Jelly Using the Osboren Classification Method |
Description | Royal jelly has long been recognized as health food, with a high content of proteins. These proteins play important roles in honeybee caste and human health, but the proteomic analysis of those low-abundance proteins in royal jelly is always a challenge. Herein, we used the Osboren classification method to separate the royal jelly proteins of Xinjiang black bees, a sub-species of Apis mellifera mellifera, into various fractions. The globulin, ethanol-soluble protein and glutelin fractions were further separated by SDS-PAGE, and proteomic analysis was carried out by LC-MS/MS and searching against the NCBI database. A total of 63 proteins with definitive names were identified, in which 41 proteins were identified for the first time in royal jelly. The Osboren classification method combined with one-dimensional gel electrophoresis based proteomic analysis allows the identification of low-abundance proteins, and greatly extends the knowledge about the components and functions of royal jelly proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-09 |
AnnouncementXML | Submission_2024-08-09_02:55:53.052.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Long Chen |
SpeciesList | scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-25 03:23:42 | ID requested | |
⏵ 1 | 2024-08-09 02:55:53 | announced | |
2 | 2024-10-22 06:53:38 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: oyal jelly |
proteome |
Osboren classification |
low-abundance protein |
Contact List
Long Chen |
contact affiliation | College of Life Science & Technology Beijing University of Chemical Technology Beijing, 10029, China |
contact email | chenlong@mail.buct.edu.cn |
lab head | |
Long Chen |
contact affiliation | Beijing University of Chemical Technology |
contact email | chenlong@mail.buct.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD023315
- Label: PRIDE project
- Name: Proteomic Analysis of Xinjiang Black Bee's Royal Jelly Using the Osboren Classification Method