PXD023311-1

PXD023311 is an original dataset announced via ProteomeXchange.

Title	Novel Proteome and N-glycoproteome of the Thermophilic Fungus Chaetomium thermophilugm in Response to High Temperature
Description	Thermophilic fungi are eukaryotic species that grow at high temperatures, but little is known about the thermophily of thermophilic fungi. Here the proteome and N-glycoproteome of Chaetomium thermophilum at varying culture temperatures (30℃, 50℃, and 55℃) were studied using hydrophilic interaction liquid chromatography enrichment and high-resolution liquid chromatography–tandem mass spectroscopy analysis. In proteome, the numbers of differentially expressed proteins were 1274, 1374, and 1063 in T50/T30, T55/T30, and T55/T50, respectively. The up-regulated proteins were involved in biological processes, such as protein folding and carbohydrate metabolism. Most down-regulated proteins were involved in molecular functions, such as structural constituent of the ribosome and structural activity. For N-glycoproteome, the numbers of differentially expressed N-glycoproteins were 160, 176, and 128 in T50/T30, T55/T30, and T55/T50, respectively. The differential glycoproteins were mainly involved in various types of N-glycan biosynthesis, mRNA surveillance pathway, and protein processing in the endoplasmic reticulum. These results indicated that an efficient protein homeostasis pathway plays an essential role in the thermophily of C. thermophilum, and N-glycosylation is involved by affecting related proteins. This is the first study to reveal thermophilic fungi's physiological response to high-temperature adaptation using omics analysis, facilitating the exploration of the thermophily mechanism of thermophilic fungi.
HostingRepository	PRIDE
AnnounceDate	2021-05-17
AnnouncementXML	Submission_2021-05-17_00:21:15.335.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jinpeng Gao
SpeciesList	scientific name: Chaetomium thermophilum; NCBI TaxID: 209285;
ModificationList	N-glycolneuraminic acid
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-25 03:22:49	ID requested
⏵ 1	2021-05-17 00:21:15	announced

Dataset with its publication pending

submitter keyword: proteome, N-glycoproteome, Chaetomium thermophilum, thermophily, glycosylation

Jinpeng Gao
contact affiliation	College of Plant Protection, Shandong Agricultural University
contact email	gaojp1991@163.com
lab head
Jinpeng Gao
contact affiliation	College of Plant Protection，Shandong Agricultural University
contact email	gaojp1991@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023311
     1. Label: PRIDE project
     2. Name: Novel Proteome and N-glycoproteome of the Thermophilic Fungus Chaetomium thermophilugm in Response to High Temperature