PXD023255-1

PXD023255 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Genetic inactivation of RIP1 kinase activity in rats protects against ischemic brain injury
Description	RIP1 kinase-mediated inflammatory and cell death pathways have been implicated in the pathology of acute and chronic disorders of the nervous system. Here, we describe a novel animal model of RIP1 kinase deficiency, generated by knock-in of the kinase-inactivating RIP1(D138N) mutation in rats. Homozygous RIP1 kinase-dead (KD) rats had normal development, reproduction and did not show any gross phenotypes at baseline. However, cells derived from RIP1 KD rats displayed resistance to necroptotic cell death. In addition, RIP1 KD rats were resistant to TNF-induced systemic shock. We studied the utility of RIP1 KD rats for neurological disorders by testing the efficacy of the genetic inactivation in the transient middle cerebral artery occlusion/reperfusion model of brain injury. RIP1 KD rats were protected in this model in a battery of behavioral, imaging and histopathological endpoints. In addition, RIP1 KD rats had reduced inflammation and accumulation of neuronal injury biomarkers. Unbiased proteomics in the plasma identified additional changes that were ameliorated by RIP1 genetic inactivation. Together these data highlight the utility of the RIP1 KD rats for target validation and biomarker studies for neurological disorders. For mass spectrometry experiment, please check the method section in the manuscript.
HostingRepository	MassIVE
AnnounceDate	2021-04-01
AnnouncementXML	Submission_2021-04-01_11:17:27.610.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meena Choi
SpeciesList	scientific name: Sprague Dawley;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-21 09:05:53	ID requested
⏵ 1	2021-04-01 11:17:27	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: DIA, Thermo Fusion Lumos, HRM, rat plasma, RIP1 kinase, ischemic brain injury

submitter keyword: DIA, Thermo Fusion Lumos, HRM, rat plasma, RIP1 kinase, ischemic brain injury

Contact List

Baris Bingol
contact affiliation	Genentech, Inc.
contact email	barisb@gene.com
lab head
Meena Choi
contact affiliation	Northeastern University
contact email	mnchoi67@gmail.com
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086623/