PXD023238-2

PXD023238 is an original dataset announced via ProteomeXchange.

Title	TENT5 cytoplasmic non-canonical poly(A) polymerases regulate the innate immune response in animals
Description	Innate immunity, the first line of host defense against pathogens, is tightly regulated both transcriptionally and post-transcriptionally. Here, through global transcriptome and proteome analyses in Caenorhabditis elegans, we uncover a modulation of the expression of secreted innate immunity effector proteins by TENT5, one of a recently described family of cytoplasmic poly(A) polymerases. Direct RNA sequencing revealed that TENT-5 polyadenylates mRNAs with signal peptide-encoding sequences, that are translated at the endoplasmic reticulum. Loss of tent-5 function makes worms more susceptible to bacterial infection. Importantly, we demonstrate that the function of TENT-5 in innate immunity is evolutionarily conserved, as its orthologs, TENT5A and TENT5C are induced during macrophage activation and polyadenylate mRNAs, some of which are of genes orthologous to C. elegans TENT-5 targets. In summary, our study reveals cytoplasmic polyadenylation to be a previously unknown component of the post-transcriptional regulation of innate immunity in animals.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:51:52.412.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominik Cysewski
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-21 02:35:31	ID requested
1	2022-05-31 02:39:52	announced
⏵ 2	2023-11-14 08:51:53	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: innate immunity, cytoplasmic polyadenylation, non-canonical polyadenylation, TENT5, direct RNA sequencing,Terminal nucleotidyltransferase, C. elegans innate immunity

Andrzej Dziembowski
contact affiliation	Laboratory of RNA Biology International Institute of Molecular and Cell Biology in Warsaw
contact email	adziembowski@iimcb.gov.pl
lab head
Dominik Cysewski
contact affiliation	Medical University of Bialystok
contact email	dominikcysewski@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD023238

PRIDE project URI

• PRIDE
  1. PXD023238
     1. Label: PRIDE project
     2. Name: TENT5 cytoplasmic non-canonical poly(A) polymerases regulate the innate immune response in animals