PXD023233-1

PXD023233 is an original dataset announced via ProteomeXchange.

Title	Bacteria in biogas digestates for reduced climate forcing
Description	Mitigation of N2O-emissions from soils is needed to reduce climate forcing by food production. Inoculating soils with N2O-reducing bacteria would be effective, but costly and impractical as a standalone operation. Here we demonstrate that digestates obtained after biogas production may provide a low-cost and widely applicable solution. Firstly, we show that indigenous N2O-reducing bacteria in digestates grow to high levels during anaerobic enrichment under N2O. Gas kinetics and meta-omic analysis show that the N2O respiring organisms, recovered as metagenome-assembled genomes (MAGs) grow by harvesting fermentation intermediates of the methanogenic consortium. Three digestate-derived denitrifying bacteria were obtained through isolation, one of which matched the recovered MAG of a dominant Dechloromonas-affiliated N2O reducer. While the identified N2O-reducers encoded genes required for a full denitrification pathway and could thus both produce and sequester N2O, their regulatory traits predicted that they act as N2O-sinks in the current system. Secondly, moving towards practical application, we show that these isolates grow by aerobic respiration in digestates, and that fertilization with these enriched digestates reduces N2O emissions. This shows that the ongoing implementation of biogas production in agriculture opens a new avenue for cheap and effective reduction of N2O emissions from food production.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_12:25:46.202.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Live Hagen
SpeciesList	scientific name: unclassified Azonexus; NCBI TaxID: 2649329;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-18 09:14:46	ID requested
⏵ 1	2022-02-16 12:25:47	announced

Jonassen KR, Hagen LH, Vick SHW, Arntzen M Ø, Eijsink VGH, Frosteg, å, rd Å, Lycus P, Molstad L, Pope PB, Bakken LR, Nitrous oxide respiring bacteria in biogas digestates for reduced agricultural emissions. ISME J, 16(2):580-590(2022) [pubmed]

submitter keyword: Anaerobic digestion, isolate, Dechloromonas sp, Azonexus sp., denitrification, N2O-reduction, LC-MSMS.

Lars Bakken
contact affiliation	Faculty of Biotechnology, Chemistry and Food Science, NMBU - Norwegian University of Life Sciences, Norway
contact email	lars.bakken@nmbu.no
lab head
Live Hagen
contact affiliation	Faculty of Biotechnology, Chemistry and Food Science, Norwegian University of Life Sciences, Norway
contact email	live.hagen@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023233
     1. Label: PRIDE project
     2. Name: Bacteria in biogas digestates for reduced climate forcing