PXD023226 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Isobaric peptide labeling on digital microfluidics for quantitative proteomics of low cell numbers is facilitated by low critical micellar concentration detergents |
Description | We developed protocols for isobaric TMT labeling on digital microfluidics (DMF) devices enabling the quantitative proteome analysis of approximately 25 mammalian cells per channel by subsequent bottom-up proteome analysis by LC-MS. This comprised identification of a compatible detergent for digestion, on-chip labeling, and LC-MS. Application of the method in a TMT 6-plex sample analysis enabled the relative quantification 648 proteins (2 unique peptides for quantification) from a total of 125 cell equivalents. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-28 |
AnnouncementXML | Submission_2022-02-28_02:51:08.437.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Andreas Tholey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter fragment; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-18 07:01:50 | ID requested | |
⏵ 1 | 2022-02-28 02:51:08 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Digital microfluidics, SP3, TMT labeling, Detergents, CMC |
Contact List
Prof. Dr. Andreas Tholey |
contact affiliation | AG Proteomics & Bioanalytics - Institut für Experimentelle Medizin Christian-Albrechts-Universität zu Kiel 24105 Kiel, Germany |
contact email | a.tholey@iem.uni-kiel.de |
lab head | |
Andreas Tholey |
contact affiliation | Systematic Proteome Research & Bioanalytics, University of Kiel |
contact email | a.tholey@iem.uni-kiel.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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[ - ]
- PRIDE
- PXD023226
- Label: PRIDE project
- Name: Isobaric peptide labeling on digital microfluidics for quantitative proteomics of low cell numbers is facilitated by low critical micellar concentration detergents