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PXD023208-1

PXD023208 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePeptides with phosphorylation sites found in human DDHD1
DescriptionFLAG-DDHD1-expressing HEK293 or PANC1 cells were treated with okadaic acid, and FLAG-DDHD1 was purified by SDS-PAGE. After the in-gel digestion of purified FLAG-DDHD1 with trypsin or Asp-N, the resultant phosphopeptides were further purified by immobilized metal affinity chromatography (IMAC) or titanium dioxide (TiO2), zirconium dioxide (ZrO2), or TiO2/ZrO2 affinity chromatography. Peptides were analyzed by MALDI-TOF MS/MS. The Mascot analysis using the database of the human Swiss-Prot or FLAG-tagged human DDHD1 sequence revealed more than 10 phosphopeptides of DDHD1 with significant scores.
HostingRepositoryjPOST
AnnounceDate2021-12-17
AnnouncementXMLSubmission_2021-12-16_07:00:05.064.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD023208
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterNaoki Matsumoto
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListO-phospho-L-serine; O-phospho-L-threonine; unknown modification
InstrumentAXIMA Performance MALDI-TOF/TOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-17 20:14:31ID requested
12021-12-16 07:00:05announced
22022-09-18 03:27:58announced2022-09-18: Updated FTP location.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: DDHD1, phosphorylation
Contact List
Naoki Matsumoto
lab head
Naoki Matsumoto
contact affiliationTeikyo university
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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