PXD023204-1

PXD023204 is an original dataset announced via ProteomeXchange.

Title	Zinc-associated proteome of Cryptococcus neoformans
Description	Fungal diseases are critical burdens on the global healthcare system, infecting over 25% of the world’s population. For the opportunistic fungal pathogen, Cryptococcus neoformans, infection leads to cryptococcosis; a disease enabled by elaboration of sophisticated virulence factors, including polysaccharide capsule, melanin, thermotolerance, and extracellular enzymes in the presence of the host. Conversely, the host protects itself from fungal invasion by regulating and sequestering transition metals (e.g., iron, zinc, copper) important for microbial growth and survival. Building upon knowledge of zinc transport regulation at the transcriptional level, here, we explore the intricate relationship between zinc availability and fungal virulence via mass spectrometry-based quantitative proteomics. We observe distinct protein-level responses to zinc-limited and -excess conditions through a shift of the proteome profile towards stress response and metal acquisition, including the upregulation of the zinc transporter, ZIP1. In addition, we reveal a novel connection among zinc availability, protein folding, capsule production, and virulence through the detection of a Wos2-like ortholog in the secretome under replete conditions. Overall, we provide new biological insight into cellular remodeling at the protein level of C. neoformans under regulated zinc conditions and uncover a novel connection between zinc availability and fungal virulence. These findings support the development of new antifungal strategies focused on the enhancement of nutritional immunity within the host.
HostingRepository	PRIDE
AnnounceDate	2022-02-16
AnnouncementXML	Submission_2022-02-16_12:29:58.359.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Geddes-McAlister
SpeciesList	scientific name: Cryptococcus neoformans var. grubii H99; NCBI TaxID: 235443;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-17 11:09:14	ID requested
⏵ 1	2022-02-16 12:29:59	announced

Ball B, Woroszchuk E, Sukumaran A, West H, Afaq A, Carruthers-Lay D, Muselius B, Gee L, Langille M, Pladwig S, Kazi S, Hendriks A, Geddes-McAlister J, Proteome and secretome profiling of zinc availability in Cryptococcus neoformans identifies Wos2 as a subtle influencer of fungal virulence determinants. BMC Microbiol, 21(1):341(2021) [pubmed]

submitter keyword: Cryptococcus neoformans, fungal pathogenesis, zinc limitation, quantitative proteomics, polysaccharide capsule, virulence

Dr. Jennifer Geddes-McAlister
contact affiliation	Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada, N1G 2W1, 519-824-4120 ext. 52129
contact email	jgeddesm@uoguelph.ca
lab head
Jennifer Geddes-McAlister
contact affiliation	University of Guelph
contact email	jgeddesm@uoguelph.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD023204

PRIDE project URI

• PRIDE
  1. PXD023204
     1. Label: PRIDE project
     2. Name: Zinc-associated proteome of Cryptococcus neoformans