PXD023200-2

PXD023200 is an original dataset announced via ProteomeXchange.

Title	Competition for electrons favors N2O reduction in denitrifying Bradyrhizobium isolates
Description	Bradyrhizobia are common members of soil microbiomes and known as N2-fixing symbionts of economically important legumes. Many are also denitrifiers, which can act as sinks or sources for N2O. Inoculation with compatible rhizobia is often needed for optimal N2-fixation, but the choice of inoculant may have consequences for N2O emission. Here, we determined the phylogeny and denitrification capacity of Bradyrhizobium strains, most of them isolated from peanut-nodules. Analyses of genomes and denitrification end-points showed that all were denitrifiers, but only ~1/3 could reduce N2O. The N2O-reducing isolates had strong preference for N2O- over NO3--reduction. Such preference was also observed in a study of other bradyrhizobia and tentatively ascribed to competition between the electron pathways to Nap (periplasmic NO3- reductase) and Nos (N2O reductase). Another possible explanation is lower abundance of Nap than Nos. Here, proteomics revealed that Nap was instead more abundant than Nos, supporting the hypothesis that the electron pathway to Nos outcompetes that to Nap. In contrast, Paracoccus denitrificans, which has membrane-bond NO3- reductase (Nar), reduced N2O and NO3- simultaneously. We propose that the control at the metabolic level, favoring N2O reduction over NO3- reduction, applies also to other denitrifiers carrying Nos and Nap but lacking Nar.
HostingRepository	PRIDE
AnnounceDate	2021-09-27
AnnouncementXML	Submission_2021-09-27_03:49:58.227.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Magnus Arntzen
SpeciesList	scientific name: Bradyrhizobium; NCBI TaxID: 374;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-17 06:17:31	ID requested
1	2021-09-27 02:09:28	announced
⏵ 2	2021-09-27 03:49:59	announced	2021-09-27: Updated project metadata.

Gao Y, Mania D, Mousavi SA, Lycus P, Arntzen M Ø, Woliy K, Lindstr, ö, m K, Shapleigh JP, Bakken LR, Frosteg, å, rd Å, O reduction in denitrifying Bradyrhizobium isolates. Environ Microbiol, 23(4):2244-2259(2021) [pubmed]

10.1111/1462-2920.15404;

submitter keyword: Denitrification, Bradyrhizobium

Åsa Helena Frostegård
contact affiliation	Faculty of Chemistry, Biotechnology and Food Science Norwegian University of Life Sciences Post Office Box 5003 1432, Ås Norway
contact email	asa.frostegard@nmbu.no
lab head
Magnus Arntzen
contact affiliation	Norwegian University of Life Sciences
contact email	magnus.arntzen@nmbu.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023200
     1. Label: PRIDE project
     2. Name: Competition for electrons favors N2O reduction in denitrifying Bradyrhizobium isolates