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PXD023121-1

PXD023121 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleFAM83F regulates canonical Wnt signalling through an interaction with CK1α
DescriptionThe function of the FAM83F protein, like the functions of many members of the FAM83 family, is poorly understood. Here we show that injection of Fam83f mRNA into Xenopus embryos causes axis duplication, a phenotype indicative of enhanced Wnt signalling. Consistent with this, overexpression of FAM83F activates Wnt signalling, whilst ablation of FAM83F from human colorectal cancer (CRC) cells attenuates it. We demonstrate that FAM83F is farnesylated and interacts and co-localises with CK1α at the plasma membrane. This interaction with CK1α is essential for FAM83F to activate Wnt signalling, and FAM83F mutants that do not interact with CK1α fail to induce axis duplication in Xenopus embryos and to activate Wnt signalling in cells. FAM83F acts upstream of GSK-3β, because the attenuation of Wnt signalling caused by loss of FAM83F can be rescued by GSK-3 inhibition. Introduction of a farnesyl-deficient mutant of FAM83F in cells through CRISPR/Cas9 genome editing redirects the FAM83F-CK1α complex away from the plasma membrane and significantly attenuates Wnt signalling, indicating that FAM83F exerts its effects on Wnt signalling at the plasma membrane.
HostingRepositoryPRIDE
AnnounceDate2020-12-23
AnnouncementXMLSubmission_2020-12-23_10:57:38.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGopal Sapkota
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListfarnesylated residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-12-14 06:44:45ID requested
12020-12-23 10:57:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: FAM83F
Contact List
Gopal Sapkota
contact affiliationMRC-PPU
contact emailg.sapkota@dundee.ac.uk
lab head
Gopal Sapkota
contact affiliationUniversity of Dundee
contact emailg.sapkota@dundee.ac.uk
dataset submitter
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Dataset FTP location
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