PXD023108 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Temporal proteomic changes induced by nicotine in human cells: A quantitative proteomics approach |
Description | Nicotine is a prominent active compound in tobacco and many smoking cessation products. Some of the biological effects of nicotine are well documented in in vitro and in vivo systems; however, nominal data are available concerning the time-dependent changes on protein and phosphorylation events in response to nicotine. Here, we profiled the proteomes of SH-SY5Y and A549 cell lines subjected to acute (15min, 1h and 4h) or chronic (24h, 48h) nicotine exposures. We used sample multiplexing (TMTpro16) and quantified more than 9,000 proteins and over 7,000 phosphorylation events per cell line. Among our findings, we determined a decrease in mitochondrial protein abundance for SH-SY5Y, while we detected alterations in several immune pathways, such as the complement system, for A549. We also explored the proposed association between smoking and SARS-CoV2. Here, we found several host proteins known to interact with viral proteins that were affected by nicotine in a time dependent manner. This dataset can be mined further to investigate the potential role of nicotine in different biological contexts. |
HostingRepository | PRIDE |
AnnounceDate | 2021-05-11 |
AnnouncementXML | Submission_2021-05-11_06:21:21.097.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Joao Paulo |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-14 01:10:57 | ID requested | |
⏵ 1 | 2021-05-11 06:21:22 | announced | |
Publication List
Navarrete-Perea J, Gygi SP, Paulo JA, Temporal proteomic changes induced by nicotine in human cells: A quantitative proteomics approach. J Proteomics, 241():104244(2021) [pubmed] |
Keyword List
submitter keyword: COVID19, FAIMS, Orbitrap Lumos, Protein-protein interactions, Real Time Search, RTS, TMTpro |
Contact List
Joao A. Paulo |
contact affiliation | Harvard Medical School, Boston, MA |
contact email | joao_paulo@post.hms.harvard.edu |
lab head | |
Joao Paulo |
contact affiliation | Harvard Medical School |
contact email | joao_paulo@post.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD023108
- Label: PRIDE project
- Name: Temporal proteomic changes induced by nicotine in human cells: A quantitative proteomics approach