PXD023096-1

PXD023096 is an original dataset announced via ProteomeXchange.

Title	: The effects of chronic morphine on the endogenous cannabinoid system in the ventral tegmental area
Description	The United States is currently facing a severe opioid epidemic, therefore addressing how opioids induce rewarding behaviors could be key to a solution for this medical and societal crisis. Recently, the endogenous cannabinoid system has emerged as a hot topic in the study of opioid reward but relatively little is known about how chronic opioid exposure may affect this system. In the present study, we investigated how chronic morphine may modulate the endogenous cannabinoid system in the ventral tegmental area (VTA), a critical region in the mesolimbic reward circuitry. Our studies found that the VTA expresses 32 different proteins or genes related to the endogenous cannabinoid system; 3 of these proteins or genes were significantly affected after chronic morphine exposure. We also investigated the effects of acute and chronic morphine treatment on the production of the primary endocannabinoids, 2-Arachidonoylglycerol (2-AG) and anandamide (AEA), and identified that acute, but not chronic, morphine treatment significantly reduced AEA production in the VTA; 2-AG levels were unchanged in either condition. Lastly, our studies exhibited a systemic enhancement of 2-AG tone via inhibition of monoacylglycerol lipase (MAGL)-mediated degradation and the pharmacological activation of cannabinoid receptor 2 (CB2R) significantly suppressed chronic morphine-induced conditioned place preference. Taken together, our studies offer a broad picture of chronic morphine-induced alterations of the VTA endogenous cannabinoid system, provide several uncharacterized targets that could be used to develop novel therapies, and identify how manipulation of the endocannabinoid system can mitigate opioid reward to directly address the ongoing opioid epidemic.
HostingRepository	PRIDE
AnnounceDate	2021-04-08
AnnouncementXML	Submission_2021-04-07_23:01:50.525.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023096
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Paul Langlais
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-13 11:28:51	ID requested
⏵ 1	2021-04-07 23:01:50	announced

Dataset with its publication pending

submitter keyword: Opioid, endogenous cannabinoid system, endocannabinoids, cannabinoid receptors, ventral tegmental area, reward

Todd Vanderah
contact affiliation	Department of Pharmacology, University of Arizona College of Medicine
contact email	vanderah@arizona.edu
lab head
Paul Langlais
contact affiliation	University of Arizona
contact email	langlais@deptofmed.arizona.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023096
     1. Label: PRIDE project
     2. Name: : The effects of chronic morphine on the endogenous cannabinoid system in the ventral tegmental area