PXD023086-2

PXD023086 is an original dataset announced via ProteomeXchange.

Title	African swine fever virus ubiquitinconjugating enzyme interacts with host translation machinery to regulate the host protein synthesis
Description	African Swine Fever virus (ASFV) causes one of the most relevant emerging diseases affecting swine, now extended through three continents. The virus has a large coding capacity to deploy an arsenal of molecules antagonizing the host functions. In the present work, we have studied the only known E2 viral-conjugating enzyme, UBCv1 that is encoded by the I215L gene of ASFV. SILAC quantitative proteomics was used toidentify and characterize novel UBCv1-host interactors. The analysis revealed interaction with the 40S ribosomal protein RPS23, the cap-dependent translation machinery initiation factor eIF4E, and the E3 ubiquitin ligase Cullin 4B. Our data show that during ASFV infection, UBCv1 was able to bind to eIF4E, independent from the cap-dependent complex. Our results provide novel insights into the function of the viral UBCv1 in hijacking cellular components that impact the mTORC signalling pathway, the regulation of the host translation machinery, and the cellular protein expression during the ASFV lifecycle. The people involved in this project are: Lucia Barrado-Gil, Covadonga Alonso and Carlos Maluquer de Motes.
HostingRepository	PRIDE
AnnounceDate	2021-01-22
AnnouncementXML	Submission_2021-01-21_23:41:07.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Carlos Maluquer de Motes
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-13 11:06:39	ID requested
1	2020-12-14 01:22:38	announced
⏵ 2	2021-01-21 23:41:08	announced	2021-01-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: ASFV

Ubiquitin conjugating enzyme

host translation

Carlos Maluquer de Motes
contact affiliation	Department of Microbial Sciences School of Biosciences and Medicine University of Surrey
contact email	c.maluquerdemotes@surrey.ac.uk
lab head
Carlos Maluquer de Motes
contact affiliation	University of Surrey
contact email	c.maluquerdemotes@surrey.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023086
     1. Label: PRIDE project
     2. Name: African swine fever virus ubiquitinconjugating enzyme interacts with host translation machinery to regulate the host protein synthesis