PXD023028-2

PXD023028 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of Plasmodium falciparum proteins interacting with SURFIN4.1
Description	Plasmodium falciparum malaria parasites export several hundred proteins to the cytoplasm of infected red blood cells (RBCs) to modify the cell environment suitable for their growth. A Plasmodium translocon of exported proteins (PTEX) is necessary for both soluble and integral membrane proteins to cross the parasitophorous vacuole membrane surrounding the parasite inside the RBC and an unidentified translocon has been proposed to have a role for integral membrane proteins to cross the parasite plasma membrane. However, the molecular composition of the translocation complex for integral membrane proteins is not well characterized. To gain insights of components of the translocation complex, we compared between tag-fused transgenic parasite lines and their controls. For the first experiment, we used a transgenic line expressing SURFIN4.1N-T-C-TyGFP (mini-SURFIN4.1, v150225v03), consisting of a short N-terminal region, a transmembrane and cytoplasmic regions of an exported integral membrane protein SURFIN4.1, a Ty-tag and GFP, to identify interacting proteins. A wild type parental parasite line (v150226v03) was used as a control. For the second experiment a transgenic line expressing mini-SURFIN4.1 (v160212v06) was compared with a line expressing TyGFP (v160216v01). For the last experiment, we used a transgenic line expressing SURFIN4.1N-T-C-BirA-HA (v150923v02), consisting of a short N-terminal region, a transmembrane and cytoplasmic regions of an exported integral membrane protein SURFIN4.1, a BirA and HA-tag, to identify interacting proteins. In this experiment a transgenic line expressing BirA*-HA (v150923v04) was used as a control.
HostingRepository	jPOST
AnnounceDate	2021-12-10
AnnouncementXML	Submission_2022-09-18_02:32:51.314.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD023028
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Osamu Kaneko
SpeciesList	scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationList	L-methionine sulfoxide; S-carboxamidomethyl-L-cysteine
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-09 18:44:32	ID requested
1	2021-12-09 07:00:12	announced
⏵ 2	2022-09-18 02:32:51	announced	2022-09-18: Updated FTP location.

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: malaria, trafficking, erythrocyte, translocation, protein complex

submitter keyword: malaria, trafficking, erythrocyte, translocation, protein complex

Contact List

Osamu Kaneko
lab head
Osamu Kaneko
contact affiliation	Department of Protozoology, Institute of Tropical Medicine, Nagasaki University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001028/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001028/