PXD023025-1

PXD023025 is an original dataset announced via ProteomeXchange.

Title	Human OCIAD2 binding partners and the effect of OCIAD2 depletion on mitochondrial protein levels
Description	Assembly of the oxidative phoshorylation dimeric complex III (CIII2) in the mitochondrial inner membrane is an intricate process in which many factors are involved. Despite many studies this process is yet to be completely understood. Here we report the identification of human OCIAD2 (Ovarian Carcinoma Immunoreactive Antigen domain containing protein 2) as an assembly factor for CIII2. OCIAD2 was found deregulated in several carcinomas and in some neurodegenerative disorders; however its non-pathological role was not elucidated to date. We have shown that OCIAD2 localizes to mitochondria and interacts with electron transport chain (ETC) proteins. Complete loss of OCIAD2 using gene editing in HEK293 cells resulted in abnormal mitochondrial morphology, decrease assembly of both CIII2 and supercomplex III2+IV and decreased activities of complex I and III. Identification of OCIAD2 as a protein required for assembly of functional CIII2 provides a new insight into the biogenesis and architecture of the ETC. Elucidating the mechanism of OCIAD2 action is important both for the understanding of cellular metabolism and for understanding of its role in the malignant transformation. This work includes two proteomic datasets that were acquired and analyzed to aid: 1) the identification of protein binding partners of OCIAD2 via co-immunoprecipitation; 2) the quantification of changes induced to protein levels in isolated mitochondria upon deletion of OCIAD2 in cells.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:44:46.728.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Remigiusz Serwa
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-09 11:21:52	ID requested
⏵ 1	2022-08-11 23:44:47	announced

Chojnacka KJ, Elancheliyan P, Mussulini BHM, Mohanraj K, Callegari S, Gosk A, Banach T, G, ó, ral T, Szczepanowska K, Rehling P, Serwa RA, Chacinska A, Ovarian carcinoma immunoreactive antigen-like protein 2 (OCIAD2) is a novel complex III-specific assembly factor in mitochondria. Mol Biol Cell, 33(4):ar29(2022) [pubmed]

submitter keyword: OCIAD2, mitochondria, oxidative phosphorylation, OxPhos Complex III

Agnieszka Chacinska
contact affiliation	ReMedy International Research Agenda Unit, University of Warsaw, Warsaw 02-097, Poland; Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland
contact email	a.chacinska@cent.uw.edu.pl
lab head
Remigiusz Serwa
contact affiliation	Centre of New Technologies, University of Warsaw
contact email	r.serwa@cent.uw.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD023025
     1. Label: PRIDE project
     2. Name: Human OCIAD2 binding partners and the effect of OCIAD2 depletion on mitochondrial protein levels