PXD023022-2

PXD023022 is an original dataset announced via ProteomeXchange.

Title	Molecular architecture of the endocytic TPLATE complex – part1 TAP/BS3 cross-linking dataset
Description	Eukaryotic cells rely on endocytosis to regulate their plasma membrane proteome and lipidome. Most eukaryotic groups, except fungi and animals, have retained the evolutionary ancient TSET complex as endocytic regulator. Unlike other coatomer complexes, structural insight into TSET is lacking. Here, we reveal the molecular architecture of plant TSET (TPLATE complex/TPC) using an integrative structural approach. We identify crucial roles for specific TSET subunits in complex assembly and membrane interaction. Our data therefore generates novel evolutionary insight into the difference between the hexameric TSET in Dictyostelium and the octameric TPC in plants. Structural elucidation of this ancient adaptor complex represents the missing piece in the coatomer-puzzle and vastly advances our functional as well as evolutionary insight into the process of endocytosis.
HostingRepository	PRIDE
AnnounceDate	2021-03-04
AnnouncementXML	Submission_2021-03-04_00:49:14.383.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dominique Eeckhout
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-09 08:28:25	ID requested
1	2021-03-04 00:27:22	announced
⏵ 2	2021-03-04 00:49:15	announced	2021-03-04: Updated project metadata.

Yperman K, Wang J, Eeckhout D, Winkler J, Vu LD, Vandorpe M, Grones P, Mylle E, Kraus M, Merceron R, Nolf J, Mor E, De Bruyn P, Loris R, Potock, ý M, Savvides SN, De Rybel B, De Jaeger G, Van Damme D, Pleskot R, Molecular architecture of the endocytic TPLATE complex. Sci Adv, 7(9):(2021) [pubmed]

10.1126/SCIADV.ABE7999;

submitter keyword: Arabidopsis, TPLATE complex, TSET, structure, BS3 cross-linking

Daniel Van Damme
contact affiliation	Ghent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium VIB Center for Plant Systems Biology, Technologiepark 71, 9052 Ghent, Belgium
contact email	daniel.vandamme@psb.vib-ugent.be
lab head
Dominique Eeckhout
contact affiliation	Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, B-9052, Belgium VIB Center for Plant Systems Biology, Ghent, B-9052, Belgium
contact email	Dominique.Eeckhout@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD023022

PRIDE project URI

• PRIDE
  1. PXD023022
     1. Label: PRIDE project
     2. Name: Molecular architecture of the endocytic TPLATE complex – part1 TAP/BS3 cross-linking dataset