PXD022987 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The Ubiquitin-Specific Protease USP36 Promotes Small Nucleolar Ribonucleoprotein Group SUMOylation and is Essential for Ribosome Biogenesis |
Description | SUMOylation plays a crucial role in regulating diverse cellular processes including ribosome biogenesis. Proteomic analyses and experimental evidence showed that a number of nucleolar proteins involved in ribosome biogenesis are modified by SUMO. However, how these proteins are SUMOylated is less understood. Here, we report that USP36, a nucleolar deubiquitinating enzyme (DUB), promotes nucleolar SUMOylation. Overexpression of USP36 enhances nucleolar SUMOylation, whereas its knockdown or genetic deletion reduces the levels of SUMOylation. USP36 interacts with SUMO2 and Ubc9 and directly mediates SUMOylation in cells and in vitro. We show that USP36 promotes the SUMOylation of the small nucleolar ribonucleoprotein (snoRNP) components Nop58 and Nhp2 in cells and in vitro and their binding to snoRNAs. It also promotes the SUMOylation of snoRNP components Nop56 and DKC1. Functionally, we show that knockdown of USP36 markedly impairs rRNA processing and translation. Thus, USP36 promotes snoRNP group SUMOylation and is critical for ribosome biogenesis and protein translation. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-29 |
AnnouncementXML | Submission_2021-03-29_15:32:13.532.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Phillip Wilmarth |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-07 23:29:51 | ID requested | |
⏵ 1 | 2021-03-29 15:32:13 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Deubiquitinating enzyme, nucleolus, Ribosome biogenesis, SUMO, SUMOylation, USP36, Homo sapiens, Spectral Count, Co-IP, LTQ Velos |
Contact List
Mu-Shui Dai |
contact affiliation | Department of Molecular & Medical Genetics Oregon Health & Science University |
contact email | daim@ohsu.edu |
lab head | |
Phillip Wilmarth |
contact affiliation | OHSU |
contact email | wilmarth@ohsu.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022987
- Label: PRIDE project
- Name: The Ubiquitin-Specific Protease USP36 Promotes Small Nucleolar Ribonucleoprotein Group SUMOylation and is Essential for Ribosome Biogenesis