PXD022954 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dietary restriction transforms the mammalian protein sulfhydrome in a tissue-specific and cystathionine γ-lyase-dependent manner |
Description | Hydrogen sulfide (H2S) is a cytoprotective redox-active metabolite that signals through protein persulfidation (R-SSnH). Despite the known importance of persulfidation on relatively few identified proteins, tissue-specific sulfhydrome profiles and their associated functions are not well characterized, specifically under conditions known to modulate H2S production. We hypothesized that dietary restriction (DR), which increases lifespan and can boost H2S production, expands tissue-specific sulfhydromes. Here, we found protein persulfidation was enriched in liver, kidney, muscle, and brain but decreased in heart of young and aged male mice under two forms of DR, with DR promoting persulfidation in numerous metabolic and aging-related pathways. Mice lacking H2S producing enzyme cystathionine γ-lyase (CGL) had overall decreased tissue protein persulfidation and failed to functionally augment sulfhydromes in response to DR. Overall, we defined tissue- and CGL-dependent sulfhydromes and how diet transforms their makeup, underscoring the breadth for DR and H2S to impact biological processes and organismal health. |
HostingRepository | PRIDE |
AnnounceDate | 2021-01-25 |
AnnouncementXML | Submission_2021-01-24_23:09:32.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD022954 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Nazmin Bithi |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | biotinylated residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-07 03:09:20 | ID requested | |
⏵ 1 | 2021-01-24 23:09:33 | announced | |
2 | 2021-04-06 02:34:19 | announced | 2021-04-06: Updated publication reference for PubMed record(s): 33741971. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: dietary restriction (DR), cystathionine γ-lyase (CGL, CSE, CTH), hydrogen sulfide (H2S), sulfhydration, persulfidation, sulfhydrome, biotin thiol assay (BTA), cysteine, every other day (EOD) fasting, longevity, redox metabolism |
Contact List
Christopher Hine |
contact affiliation | Cleveland Clinic Lerner Research Institute Cardiovascular and Metabolic Sciences |
contact email | hinec@ccf.org |
lab head | |
Nazmin Bithi |
contact affiliation | Cleveland Clinic Lerner Research Institute |
contact email | bithin@ccf.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
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- PRIDE
- PXD022954
- Label: PRIDE project
- Name: Dietary restriction transforms the mammalian protein sulfhydrome in a tissue-specific and cystathionine γ-lyase-dependent manner