PXD022952 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Protein profiling reveals the characteristic changes of complement cascade pathway in the tissues of gastric signet ring cell carcinoma |
Description | Signet ring cell carcinoma (SRCC) is a histological subtype of gastric cancer with distinct features in multiple aspects compared to adenocarcinomas (ACs). Lacking of systematically molecular overview to this disease made a slow progress in its clinical practice. In the present proteomics study, gastric tissues were collected from tumor and adjacency including 14 SRCCs and 34 ACs, and laser capture microdissection (LCM) was employed to eradicate cellular heterogeneity of the tissues. Proteome of tissues were profiled by data independent acquisition (DIA) mass spectrometry (MS). Based on the over 6 000 proteins quantified. Univariate analysis and pathway enrichment revealed that some proteins and pathways bared the differences between SRCC and ACs. Importantly, the up regulation for a majority of complement-related proteins were iconic for SRCC but not for AC. A hypothesis, based on the proteomics evidence, was proposed that the complement cascade was evoked in the SRCC microenvironment upon infiltration, while the SRCC cells survived the complement cytotoxicity by secreting endogenous negative regulators. Moreover, an attempt was made to seek appropriate cell models for gastric SRCC, through proteomic comparison of the 15 gastric cell lines and the gastric tumors. The prediction of supervised classifier suggested none of these gastric cell lines qualified in mimicking SRCC. This study discovered that complement cascade is activated at a higher level in gastric SRCC than AC. |
HostingRepository | PRIDE |
AnnounceDate | 2021-02-25 |
AnnouncementXML | Submission_2021-02-24_23:50:50.672.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | yuting liang |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-07 03:09:06 | ID requested | |
⏵ 1 | 2021-02-24 23:50:51 | announced | |
2 | 2021-03-14 23:05:56 | announced | 2021-03-15: Updated publication reference for PubMed record(s): 33676000. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Stomach Neoplasms, Signet Ring Cell, Mass Spectrometry, Proteomics, Complement System Proteins |
Contact List
Yan Ren |
contact affiliation | Clinical Laboratory of BGI Health, BGI-Shenzhen, Shenzhen 518083, China |
contact email | reny@bgi.com |
lab head | |
yuting liang |
contact affiliation | BGI |
contact email | 1471800169@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022952
- Label: PRIDE project
- Name: Protein profiling reveals the characteristic changes of complement cascade pathway in the tissues of gastric signet ring cell carcinoma