PXD022941-1

PXD022941 is an original dataset announced via ProteomeXchange.

Title	Stradard protein and yeast top-down data
Description	We developed a novel dissociation strategy in the data acquisition process, the Mesh strategy to dissociate multiple charge states of one proteoform with stepped HCD (high collision energy dissociation). We proved that the ‘Mesh’ strategy could generate fragments with improved sequence coverage and it could also improve identification rate in top-down proteomics analysis for complicated samples. The strategy is implemented within an an open source real-time instrument control software program called MetaDrive to perform real time deconvolution and precursor selection.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_03:33:11.588.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Lu
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	dehydrated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-07 00:04:36	ID requested
⏵ 1	2022-06-09 03:33:12	announced

Lu L, Scalf M, Shortreed MR, Smith LM, Mesh Fragmentation Improves Dissociation Efficiency in Top-down Proteomics. J Am Soc Mass Spectrom, 32(6):1319-1325(2021) [pubmed]

submitter keyword: top-down, real-time, instrument control, Mesh, stepped HCD

Lei Lu
contact affiliation	Dr. Lloyd Smith lab, Chemistry Department, University of Wisconsin-Madison
contact email	llu56@wisc.edu
lab head
Lei Lu
contact affiliation	University of Wisconsin, Madison
contact email	llu56@wisc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/06/PXD022941

PRIDE project URI

• PRIDE
  1. PXD022941
     1. Label: PRIDE project
     2. Name: Stradard protein and yeast top-down data