PXD022932-1
PXD022932 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Impaired phosphatidylethanolamine metabolism activates a reversible stress response that detects and resolves mutant mitochondrial precursors |
| Description | For decades, the endoplasmic reticulum (ER)-resident tri-methylation pathway of phosphatidylcholine production that is fed by phosphatidylethanolamine (PE) made in the mitochondrion, served as a phospholipid trafficking paradigm. Recently, the strict mitochondrial localization of the enzyme that makes PE in the mitochondrion, phosphatidylserine decarboxylase 1 (Psd1), was questioned. Since a dual localization of Psd1 to the ER would have far-reaching implications, we initiated our study to independently re-assess the subcellular distribution of Psd1. Our results support the unavoidable conclusion that the vast majority, if not all, of functional Psd1 resides in the mitochondrion. Through our efforts, we discovered that mutant forms of Psd1 that impair a self-processing step needed for it to become functional, are dually localized to the ER when expressed in a PE-limiting environment. We conclude that severely impaired cellular PE metabolism provokes an ER-assisted adaptive response that is capable of identifying and resolving nonfunctional mitochondrial precursors. |
| HostingRepository | MassIVE |
| AnnounceDate | 2021-02-01 |
| AnnouncementXML | Submission_2021-02-01_08:11:51.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jonathan Trinidad |
| SpeciesList | scientific name: Saccharomyces cerevisiae; common name: baker's yeast; NCBI TaxID: 4932; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Orbitrap Fusion Lumos |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-12-04 08:36:39 | ID requested | |
| ⏵ 1 | 2021-02-01 08:11:52 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: endoplasmic reticulum phosphatidylethanolamine mitochondria Psd1 metabolism self-processing |
Contact List
| Steven M. Claypool | |
|---|---|
| contact affiliation | Johns Hopkins University School of Medicine |
| contact email | sclaypo1@jhmi.edu |
| lab head | |
| Jonathan Trinidad | |
| contact affiliation | Indiana University |
| contact email | trinidad@indiana.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086558/ |
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