PXD022915-2

PXD022915 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of gastric cancer patients with diabetes mellitus
Description	Proteomics is a powerful approach to study the molecular mechanisms of cancer. In this study, we aim to characterize the proteomic profile of gastric cancer (GC) from patients with diabetes mellitus (DM). Forty gastric cancer tissue samples including 19 cases from diabetic patients, and 21 cases from individuals without diabetes (control group), were selected for proteomics analysis. Gastric tissues were lysed, sonicated and the resulting protein content was determined. One hundred g of proteins from each sample were processed following the single-pot, solid-phase-enhanced sample preparation approach – SP3 and enzymatically digested with trypsin. The resulting peptides were analyzed by LC-MS mass spectrometry. Comparison of protein expression levels between GC samples from diabetic and non-diabetic patients was performed by label-free quantification - LFQ. A total of 6599 protein groups were identified among the 40 samples. Two hundred-twenty proteins with at least two unique peptides were differentially expressed among the two groups (adjusted p-value < 0.05), 102 being upregulated and 118 downregulated in the diabetic cohort. Statistical overrepresentation tests were considered for different annotation sets including GO, KEGG, Reactome including Disease functional databases. Upregulated proteins in GC samples from diabetic patients showed a strong fold enrichment associated with respiratory electron transport and alcohol metabolic biological processes, while downregulated proteins were associated with cell-cell junction cellular components. Six proteins were exclusively detected in GC samples from diabetic patients, while 11 proteins were found to be absent in this group.
HostingRepository	PRIDE
AnnounceDate	2021-01-22
AnnouncementXML	Submission_2021-02-08_22:19:50.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022915
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hugo Osorio
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-04 03:03:56	ID requested
1	2021-01-21 23:46:03	announced
⏵ 2	2021-02-08 22:19:51	announced	2021-02-09: Updated publication reference for PubMed record(s): 33494396.
3	2021-03-17 05:11:53	announced	2021-03-17: Updated project metadata.
4	2021-03-18 03:21:19	announced	2021-03-18: Updated project metadata.

Os, ó, rio H, Silva C, Ferreira M, Gullo I, M, á, ximo V, Barros R, Mendon, ç, a F, Oliveira C, Carneiro F, Proteomics Analysis of Gastric Cancer Patients with Diabetes Mellitus. J Clin Med, 10(3):(2021) [pubmed]

submitter keyword: gastric cancer (GC)

diabetes mellitus (DM)

proteomics

label free quantification (LFQ)

Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto, Portugal
contact email	hosorio@i3s.up.pt
lab head
Hugo Osorio
contact affiliation	i3S - Institute for Research and Innovation in Health, University of Porto
contact email	hosorio@i3s.up.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD022915

PRIDE project URI

• PRIDE
  1. PXD022915
     1. Label: PRIDE project
     2. Name: Proteomics analysis of gastric cancer patients with diabetes mellitus