PXD022913-1

PXD022913 is an original dataset announced via ProteomeXchange.

Title	TALEN-based HvMPK3 knockout attenuates proteome and phenotype responses to flg22 in barley
Description	We investigated proteome and the involvement of barley MPK3 in response to flagellin peptide flg22, a well-known bacterial elicitor. We show that MPK3 knock out lines of barley (Hv MPK3 KO) exhibit constitutive downregulation of defence related proteins such as PR proteins belonging to thaumatin family and chitinases. Further proteomic analyses showed, that the same protein families were less prone to flagellin elicitation in MPK3 KO plants compared to wild types. These results positively correlated with chitinase activity analyses. Differential proteome also indicated possible root growth defects in the mutant plants, which was corroborated with early root growth retardation and altered short- and mid-term reactions to flg22 in terms of radial root expansion and root hair emergence.
HostingRepository	PRIDE
AnnounceDate	2021-05-03
AnnouncementXML	Submission_2021-05-02_22:47:04.401.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tibor Pechan
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-04 03:03:43	ID requested
⏵ 1	2021-05-02 22:47:04	announced

Dataset with its publication pending

submitter keyword: flagellin, barley, HvMPK3, proteomics, root hairs, PR proteins, chitinases

Tibor Pechan
contact affiliation	Institute for Genomics, Biocomputing and Biotechnology Mississippi State University
contact email	pechan@ra.msstate.edu
lab head
Tibor Pechan
contact affiliation	IGBB, Mississippi State University
contact email	pechan@ra.msstate.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022913
     1. Label: PRIDE project
     2. Name: TALEN-based HvMPK3 knockout attenuates proteome and phenotype responses to flg22 in barley