PXD022909 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | TLR9-activated B cells imprint adoptively transferred CD8+ T cells with potent tumor immunity and persistence |
Description | Here we report a new way to reverse the tolerant state of adoptively transferred CD8+ T cells against melanoma through ex vivo expansion with the TLR9 agonist CpG. CpG-generated T cells elicited potent immunity without co-administration of high dose IL-2 or vaccination, which are adjuvants classically required to effectively treat solid tumors. CpG-expanded T cells exhibited an IL-2RhighICOShighCD39low phenotype ex vivo and engrafted robustly in vivo. In culture, B cells were the only cell type essential for imprinting T cells with this phenotype and potent tumor immunity. CpG agonists targeting B cells, but not dendritic cells, generated CD8+ T cell products with remarkable antitumor properties. Purified B cells were sufficient to mediate the CpG-associated changes in T cells. These findings reveal a vital role for B cells in the generation of effective antitumor T cells and have immediate implications for profoundly improving immunotherapy for patients. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-22 |
AnnouncementXML | Submission_2022-02-22_04:25:45.983.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jennifer Bethard |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-12-04 03:03:20 | ID requested | |
⏵ 1 | 2022-02-22 04:25:48 | announced | |
Publication List
Smith AS, Knochelmann HM, Wyatt MM, Rangel Rivera GO, Rivera-Reyes AM, Dwyer CJ, Ware MB, Cole AC, Neskey DM, Rubinstein MP, Liu B, Thaxton JE, Bartee E, Paulos CM, T cells with enhanced tumor immunity. J Immunother Cancer, 10(1):(2022) [pubmed] |
Keyword List
submitter keyword: T cells Adoptive cell transfer therapy Toll-like receptors melanoma B cells |
Contact List
Chrystal Paulos PhD |
contact affiliation | Medical University of South Carolina College of Medicine Department of Microbiology and Immunology Acting Associate Professor, Department of Surgery, Department of Microbiology and Immunology Emory University School of Medicine Director Translational Research for Cutaneous Malignancies Winship Cancer Institute of Emory University |
contact email | chrystal.mary.paulos@emory.edu |
lab head | |
Jennifer Bethard |
contact affiliation | Medical University of SC |
contact email | bethard@musc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022909
- Label: PRIDE project
- Name: TLR9-activated B cells imprint adoptively transferred CD8+ T cells with potent tumor immunity and persistence