PXD022864-2

PXD022864 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling of plasma membrane proteins from bovine rod photoreceptor outer segments
Description	The outer segment (OS) organelle of vertebrate photoreceptors is a highly specialized cilium whose plasma membrane plays vital and diverse roles in supporting photoreceptor function and health. However, little is known about the identity of its protein constituents, as this membrane cannot be purified to homogeneity. In this study, we employed the technique of protein correlation profiling to identify unique OS plasma membrane proteins. To achieve this, we used label-free quantitative mass spectrometry to compare relative protein abundances in an enriched preparation of the OS plasma membrane to a preparation of total outer segment membranes. We have found that only five proteins were enriched at the same level as previously validated OS plasma membrane markers. Two of these proteins, TMEM67 and TMEM237, had not been previously assigned to this membrane and one, embigin, had not been identified in photoreceptors. We further showed that embigin associates with monocarboxylate transporter MCT1 in the OS plasma membrane, facilitating lactate transport through this cellular compartment.
HostingRepository	PRIDE
AnnounceDate	2021-04-30
AnnouncementXML	Submission_2021-05-06_23:11:41.076.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022864
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nikolai Skiba
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2020-12-02 02:46:15	ID requested
1	2021-04-30 12:24:11	announced
⏵ 2	2021-05-06 23:11:41	announced	2021-05-07: Updated publication reference for PubMed record(s): 33933680.

Skiba NP, Cady MA, Molday L, Han JYS, Lewis TR, Spencer WJ, Thompson WJ, Hiles S, Philp NJ, Molday RS, Arshavsky VY, TMEM67, TMEM237, and Embigin in Complex With Monocarboxylate Transporter MCT1 Are Unique Components of the Photoreceptor Outer Segment Plasma Membrane. Mol Cell Proteomics, 20():100088(2021) [pubmed]

submitter keyword: retina, photoreneptor, outer segment, protein correlation profiling

Vadim Arshavsky
contact affiliation	Duke University Eye Center
contact email	vadim.arshavsky@duke.edu
lab head
Nikolai Skiba
contact affiliation	Duke University
contact email	nikolai.skiba@duke.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/04/PXD022864

PRIDE project URI

• PRIDE
  1. PXD022864
     1. Label: PRIDE project
     2. Name: Proteomic profiling of plasma membrane proteins from bovine rod photoreceptor outer segments