PXD022821-1

PXD022821 is an original dataset announced via ProteomeXchange.

Title	The proteomic response of the coral symbiont Symbiodinium microadriaticum to thermal and nutrient stress
Description	Abstract The coral–dinoflagellate symbiosis is increasingly disrupted by global and local anthropogenic stressors. Coral bleaching is primarily a result of high sea surface temperatures, while eutrophication is associated with reef ecosystem degradation. Excess inorganic nitrogen relative to phosphate has been proposed to directly sensitise corals to thermal bleaching and accelerate reef decline. We assessed the proteomic response of the dinoflagellate coral symbiont Symbiodinium microadriaticum to elevated temperatures under multiple nutrient conditions by mass spectrometry. Elevated temperatures resulted in reductions of many chloroplast proteins, particularly light-harvesting complexes, with simultaneous increases in chaperone proteins. N:P imbalance had a larger effect on the proteome than temperature, but the biological processes and proteins responding to each stressor largely overlapped. The proteomes were highly similar at low N:P ratios but were strongly affected by phosphate starvation. High N:P ratios inhibited cell division, reflected by changes in proteins involved in protein translation. Imbalanced N:P did not increase sensitivity to high temperatures as measured by physiological means; however, imbalanced N:P strongly upregulated cell redox homeostasis proteins at high temperatures. As redox balance is critical during thermal bleaching, these data provide insight into the mechanisms of cellular responses to thermal and multiple stresses in the coral–dinoflagellate symbiosis.
HostingRepository	PRIDE
AnnounceDate	2023-01-31
AnnouncementXML	Submission_2023-01-30_19:12:54.314.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	ClintonOakley
SpeciesList	scientific name: Symbiodinium microadriaticum; NCBI TaxID: 2951;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-30 03:59:28	ID requested
⏵ 1	2023-01-30 19:12:54	announced
2	2023-11-14 08:51:32	announced	2023-11-14: Updated project metadata.

submitter keyword: nutrient stress, thermal stress, coral reefs, coral bleaching,Symbiodinium

Simon KeithDavy
contact affiliation	School of Biological Sciences, Victoria University of Wellington, New Zealand
contact email	simon.davy@vuw.ac.nz
lab head
ClintonOakley
contact affiliation	Victoria University of Wellington
contact email	clint.oakley@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/01/PXD022821

PRIDE project URI

• PRIDE
  1. PXD022821
     1. Label: PRIDE project
     2. Name: The proteomic response of the coral symbiont Symbiodinium microadriaticum to thermal and nutrient stress