PXD022811-1

PXD022811 is an original dataset announced via ProteomeXchange.

Title	SOX4 AND SMARCA4 COOPERATIVELY REGULATE PI3K SIGNALING THROUGH TRANSCRIPTIONAL ACTIVATION OF TGFBR2 IN BASAL LIKE BREAST CANCER
Description	Dysregulation of PI3K/Akt signaling is a dominant feature in basal-like or triple-negative breast cancers (TNBC). However, the mechanisms regulating this pathway are largely unknown in this subset of aggressive tumors. Here we demonstrate that the transcription factor SOX4 is a key regulator of PI3K signaling in TNBC. Genomic and proteomic analyses coupled with mechanistic studies identified TGFBR2 as a direct transcriptional target of SOX4 and demonstrated that TGFBR2 is required to mediate SOX4-dependent PI3K signaling. We further report that SOX4 and the SWI/SNF ATPase SMARCA4, which are uniformly overexpressed in basal-like tumors, form a previously unreported complex that is required to maintain an open chromatin conformation at the TGFBR2 regulatory regions in order to mediate TGFBR2 expression and PI3K signaling. Collectively, our findings delineate the mechanism by which SOX4 and SMARCA4 cooperatively regulate PI3K/Akt signaling and suggest that this complex may play an essential role in TNBC genesis and/or progression.
HostingRepository	PRIDE
AnnounceDate	2021-03-02
AnnouncementXML	Submission_2021-03-02_09:25:36.919.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD022811
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Gaurav Mehta
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-30 03:58:37	ID requested
⏵ 1	2021-03-02 09:25:37	announced

Dataset with its publication pending

submitter keyword: SOX4, TGFBR2, SMARCA4, SWI/SNF, PI3 kinase, transcription, chromatin, epigenetics, breast cancer

Michael L Gatza
contact affiliation	Michael L. Gatza, PhD Principal Investigator Assistant Professor of Radiation Oncology Robert Wood Johnson Medical School Rutgers Cancer Institute of New Jersey Rutgers, The State University of New Jersey
contact email	mlg246@cinj.rutgers.edu
lab head
Gaurav Mehta
contact affiliation	Rutgers Cancer Institute of New Jersey - Rutgers University
contact email	gam160@cinj.rutgers.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD022811

PRIDE project URI

• PRIDE
  1. PXD022811
     1. Label: PRIDE project
     2. Name: SOX4 AND SMARCA4 COOPERATIVELY REGULATE PI3K SIGNALING THROUGH TRANSCRIPTIONAL ACTIVATION OF TGFBR2 IN BASAL LIKE BREAST CANCER