PXD022805-1

PXD022805 is an original dataset announced via ProteomeXchange.

Title	OXPHOS deficiency activates global adaptations to maintain mitochondrial membrane potential
Description	Reduction of mitochondrial membrane potential is a hallmark of mitochondrial dysfunction. It activates adaptive responses in organisms from yeast to human to rewire metabolism, remove depolarized mitochondria, and degrade unimported precursor proteins. It remains unclear how cells maintain mitochondrial membrane potential, which is critical for maintaining iron-sulfur cluster (ISC) synthesis, an indispensable function of mitochondria. Here we show that yeast oxidative phosphorylation mutants deficient in complex III, IV, V, and mtDNA respectively, have graded reduction of mitochondrial membrane potential and proliferation rates. Extensive omics analyses of these mutants show that accompanying mitochondrial membrane potential reduction, these mutants progressively activate adaptive responses, including transcriptional downregulation of ATP synthase inhibitor Inh1 and OXPHOS subunits, Puf3-mediated upregulation of import receptor Mia40 and global mitochondrial biogenesis, Snf1/AMPK-mediated upregulation of glycolysis and repression of ribosome biogenesis, and transcriptional upregulation of cytoplasmic chaperones. These adaptations disinhibit mitochondrial ATP hydrolysis, remodel mitochondrial proteome, and optimize ATP supply to mitochondria to convergently maintain mitochondrial membrane potential, ISC biosynthesis, and cell proliferation.
HostingRepository	PRIDE
AnnounceDate	2021-01-19
AnnouncementXML	Submission_2021-01-19_00:03:28.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Siqi Liu
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; 6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-30 02:15:43	ID requested
⏵ 1	2021-01-19 00:03:29	announced

Dataset with its publication pending

submitter keyword: Saccharomyces cerevisiae, Q Exactive mass spectrometer, LTQ Orbitrap Velos

Hui Jiang
contact affiliation	National Institute of Biological Sciences
contact email	jianghui@nibs.ac.cn
lab head
Siqi Liu
contact affiliation	National institute of biological sciences
contact email	liusiqi@nibs.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022805
     1. Label: PRIDE project
     2. Name: OXPHOS deficiency activates global adaptations to maintain mitochondrial membrane potential