PXD022782 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mast cell-derived SAMD14 is a novel regulator of the human prostate tumor microenvironment |
Description | Mast cells (MCs) are important cellular components of the tumor microenvironment and are significantly associated with poor patient outcomes in prostate cancer and other solid cancers. The promotion of tumor progression partly involves heterotypic interactions between MCs and cancer-associated fibroblasts (CAFs) which combine to potentiate a pro-tumor extracellular matrix and promote epithelial cell invasion and migration. Thus far, the interactions between MCs and CAFs remains poorly understood. To identify molecular changes that may alter resident MC function in the prostate tumor microenvironment, we profiled the transcriptome of human prostate MCs, isolated from patient-matched non-tumor and tumor-associated regions of fresh radical prostatectomy tissue. Transcriptomic profiling revealed a distinct gene expression profile of MCs isolated from prostate tumor regions, including the downregulation of SAMD14, a putative tumor suppressor gene. Proteomic profiling revealed overexpression of SAMD14 in HMC-1 MCs altered the secretion of proteins associated with immune regulation and extracellular matrix processes. To assess MC biological function within a model of the prostate tumor microenvironment, HMC-1-SAMD14+ conditioned media was added to co-cultures of primary prostatic CAFs and prostate epithelium. HMC-1-SAMD14+ secretions were shown to reduce the deposition and alignment of matrix produced by CAFs and suppress pro-tumorigenic prostate epithelial morphology. Overall, our data presents the first profile of human MCs derived from patient prostate cancer specimens and identifies MC-derived SAMD14 as an important mediator of MC phenotype and function within the prostate tumor microenvironment. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-12 |
AnnouncementXML | Submission_2021-03-11_22:26:34.886.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ralf Schittenhelm |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-27 06:08:30 | ID requested | |
⏵ 1 | 2021-03-11 22:26:35 | announced | |
2 | 2021-04-06 23:20:17 | announced | 2021-04-07: Updated publication reference for PubMed record(s): 33799802. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Prostate cancer |
tumor microenvironment |
mast cells |
SAMD14 |
cancer-associated fibroblasts |
extracellular matrix |
Contact List
Natalie Lister |
contact affiliation | Monash Partners Comprehensive Cancer Consortium, Monash Biomedicine Discovery Institute Cancer Program, Prostate Cancer Research Group, Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria 3800, Australia |
contact email | natalie.lister@monash.edu |
lab head | |
Ralf Schittenhelm |
contact affiliation | Monash University |
contact email | ralf.schittenhelm@monash.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022782
- Label: PRIDE project
- Name: Mast cell-derived SAMD14 is a novel regulator of the human prostate tumor microenvironment