PXD022763-1

PXD022763 is an original dataset announced via ProteomeXchange.

Title	Conserved function of UPF0016 manganese transporter across human, plant and bacteria
Description	The Uncharacterized Protein Family 0016 (UPF0016) is conserved throughout evolution. In humans, the member TMEM165 is a putative Golgi localized Ca2+ and/or Mn2+ transporter. Genes encoding UPF0016 proteins are also present in many bacteria, including the cyanobacteria Synechocystis (MNX/SynPAM71), where it has been described as Mn2+ exporter. In Arabidopsis, two members of this family are embedded in the chloroplast membranes; the CMT1 protein is localized in the inner envelope membrane and the PAM71 protein is localized in the thylakoid membrane, and both cooperate to transport Mn2+ to the oxygen evolving complex of photosystem II. We used the pam71 mutant of Arabidopsis as a platform to perform intra- and inter-species complementation assays. The N-terminus of PAM71 was fused in front of TMEM165, CMT1 and MNX to direct them into chloroplast membranes. We performed LC-MS/MS experiments to assess their sub-plastidial localization.
HostingRepository	PRIDE
AnnounceDate	2021-04-20
AnnouncementXML	Submission_2021-04-20_02:10:01.804.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giada Marino
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	impact II

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-26 03:18:00	ID requested
⏵ 1	2021-04-20 02:10:02	announced

Dataset with its publication pending

submitter keyword: Arabidopsis, ImpactII, chloroplast, thylakoid, UPF0016, Mn transporter

Anja Schneider
contact affiliation	Ludwig-Maximilians-University Munich (LMU), Department Biology I, Großhaderner Str. 2, D-82152 Planegg-Martinsried, Germany
contact email	anja.schneider@lrz.uni-muenchen.de
lab head
Giada Marino
contact affiliation	Ludwig-Maximilians-University Munich (LMU) Department Biology I Plant Molecular Biology (Botany)
contact email	giada.marino@lmu.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022763
     1. Label: PRIDE project
     2. Name: Conserved function of UPF0016 manganese transporter across human, plant and bacteria