PXD022749-1
PXD022749 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Differential proteomic analysis reveals the mechanism of Providencia.sp to selenium and manganese stress |
Description | In this study, iTRAQ technique is employed to reveal the proteomic response of Providencia. sp under selenium and manganese stress. After trypsin digestion, peptide was desalted by Strata X C18 SPE column (Phenomenex) and vacuum-dried. Peptide was reconstituted in 0.5 M TEAB and processed according to the manufacturer’s protocol for TMT kit/iTRAQ kit. Then, The tryptic peptides were fractionated into fractions by high pH reverse-phase HPLC using Agilent 300Extend C18 column. Finally, The peptides were subjected to NSI source followed by tandem mass spectrometry (MS/MS) in Q ExactiveTM Plus (Thermo) coupled online to the UPLC. |
HostingRepository | iProX |
AnnounceDate | 2020-11-26 |
AnnouncementXML | Submission_2020-11-25_22:16:10.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | ShengWei Huang |
SpeciesList | scientific name: Providencia rettgeri 947; NCBI TaxID: 1255575; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2020-11-25 22:14:39 | ID requested | |
⏵ 1 | 2020-11-25 22:16:10 | announced |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Providencia. sp, selenium, manganese, iTRAQ |
Contact List
LiFang wu | |
---|---|
contact affiliation | Hefei Institutes of Physical Science, Chinese Academy of Sciences |
contact email | lfwu@ipp.ac.cn |
lab head | |
ShengWei Huang | |
contact affiliation | Hefei Institutes of Physical Science, Chinese Academy of Sciences |
contact email | swhuang@ipp.ac.cn |
dataset submitter |
Full Dataset Link List
iProX dataset URI |