PXD022742-1
PXD022742 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Anaerobic microbial metabolism of dichloroacetate |
| Description | Anaerobic degradation of dichloroacetate (DCA) in the environment is not well understood. Recently, a microbial mixed culture (RM) was derived from pristine freshwater sediment enriched with dichloromethane (CH2Cl2, DCM) as the sole energy source under anoxic conditions. RM comprises of Candidatus Dichloromethanomonas elyunquensis strain RM which cannot only utilize DCM as an energy source, but also DCA, with the transient formation of formate, H2, and carbon monoxide (CO). To understand the metabolic capabilities of Ca. Dichloromethanomonas elyunquensi, a comparative global proteomic analysis between DCA-grown (MS datasets 1,2,3 - T8,T15) and DCM-grown (MS datasets 4,5,6 - T12,T15) cells was performed. Differential protein expression analysis identified enzymes catalyzing the conversion of DCA to acetyl-coenzyme A (CoA) via glyoxylate as well as enzymes of the Wood-Ljungdahl pathway in DCA fermentation. Combined with physiological, biochemical, and genomics information, these results demonstrate the involvement of a haloacid dehalogenase and the Wood-Ljungdahl pathway in the anaerobic metabolism of DCA, which has broader implications in our understanding of DCA turnover in natural and engineered environments. |
| HostingRepository | MassIVE |
| AnnounceDate | 2020-11-25 |
| AnnouncementXML | Submission_2020-11-25_11:57:42.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Manuel Villalobos Solis |
| SpeciesList | scientific name: Candidatus Dichloromethanomonas elyunquensis strain RM; |
| ModificationList | Oxidation; CarbamidomethylDTT |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-11-25 11:31:31 | ID requested | |
| ⏵ 1 | 2020-11-25 11:57:43 | announced |
Publication List
| no publication |
Keyword List
| submitter keyword: Dichloroacetate, haloacid dehalogenase, fermentation, comparative proteomics, metabolic pathway |
Contact List
| Frank E. Loffler | |
|---|---|
| contact affiliation | University of Tennessee-Knoxville |
| contact email | frank.loeffler@utk.edu |
| lab head | |
| Manuel Villalobos Solis | |
| contact affiliation | University of Tennessee/Oak Ridge National Laboratory |
| contact email | mvillal1@vols.utk.edu |
| dataset submitter | |
Full Dataset Link List
| MassIVE dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000086520/ |
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