PXD022741-2

PXD022741 is an original dataset announced via ProteomeXchange.

Title	Categorization of Phosphorylation Site Behavior During the Diauxic Shift in Saccharomyces cerevisiae
Description	Protein phosphorylation has long been recognized as an essential regulator of protein activity, structure, complex formation, and sub-cellular localization among other cellular mechanisms. However, interpretation of the changes in protein phosphorylation is difficult. To address this difficulty, we measured protein and phosphorylation site changes across 11 points of a time course and developed a method for categorizing phosphorylation site behavior relative to protein level changes using the diauxic shift in yeast as a model and TMT11 sample multiplexing. We classified quantified proteins into behavioral categories that reflected differences in kinase activity, protein complex structure, and growth and metabolic pathway regulation across different phases of the diauxic shift. These data also provide a valuable resource for the study of fermentative versus respiratory growth and set a new benchmark for temporal quantitative proteomics and phosphoproteomics for Diauxic Shift in Saccharomyces cerevisiae.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:19:42.533.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brandon Gassaway
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-25 08:37:48	ID requested
1	2021-03-04 01:02:00	announced
⏵ 2	2024-10-22 05:19:48	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.0c00943;

Gassaway BM, Paulo JA, Gygi SP, . J Proteome Res, 20(5):2487-2496(2021) [pubmed]

submitter keyword: Phosphoproteomics,Yeast, Diauxic Shift

Steven Gygi
contact affiliation	Department of Cell Biology, Harvard Medical School
contact email	steven_gygi@hms.harvard.edu
lab head
Brandon Gassaway
contact affiliation	Harvard Medical School
contact email	brandon_gassaway@hms.harvard.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022741
     1. Label: PRIDE project
     2. Name: Categorization of Phosphorylation Site Behavior During the Diauxic Shift in Saccharomyces cerevisiae