PXD022712 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Integrative analysis of two russeted and non-russeted closely related apple varieties during fruit development provides new insights about the cuticle/suberin interplay |
Description | Apple russeting is characterized by the deposition of suberin in the inner part of the epidermal cell walls. It develops at the fruit surface when the integrity of the cuticle has been lost. Cutin and wax composition (including triterpene profile) is also affected in russeted skin. In the present work, the fruit growth kinetic of two closely related apple varieties, ‘Canada Gris’ and ‘Canada Blanc’, which display russeted and waxy skin phenotypes, respectively, was investigated. We used a combination of transcriptomic, proteomic, and metabolomic approaches to better understand the molecular events associated with cuticle failure and suberization. A particular interest was also given to the triterpene specifically found in russeted skins and their synthesis. Our results indicate that the decreased synthesis of cutin and wax, observed in russeted skin throughout fruit development, is a determining factor for russeting. We identified a lipid transfer protein (LTP-3) as a potential key player in cuticle formation. Metabolomics highlighted a large diversity of triterpene-hydroxycinnamates in russeted tissues whose accumulation was highly correlated with suberin-related genes, including some enzymes belonging to the BAHD (HXXXD-motif) acyltransferase family. This study shed light for the first time on the crosstalk between triterpene and suberin pathways. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-22 |
AnnouncementXML | Submission_2022-02-22_04:20:27.969.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Céline LECLERCQ |
SpeciesList | scientific name: Malus domestica; NCBI TaxID: 3750; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetic acid derivatized residue |
Instrument | TripleTOF 5600+ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-24 09:04:36 | ID requested | |
⏵ 1 | 2022-02-22 04:20:28 | announced | |
Publication List
Andr, é CM, Guerriero G, Lateur M, Charton S, Leclercq CC, Renaut J, Hausman JF, Legay S, Identification of Novel Candidate Genes Involved in Apple Cuticle Integrity and Russeting-Associated Triterpene Synthesis Using Metabolomic, Proteomic, and Transcriptomic Data. Plants (Basel), 11(3):(2022) [pubmed] |
Keyword List
submitter keyword: : russeting, integrative analysis, LC-MS/MS, metabolomics, conjugated triterpene, suberin, apple skin. |
Contact List
Céline LECLERCQ |
contact affiliation | LIST-ERIN-ERTP-Green Tech platform Belvaux, LUXEMBOURG |
contact email | celine.leclercq@list.lu |
lab head | |
Céline LECLERCQ |
contact affiliation | LIST-ERIN-ERTP-GreenTech platform |
contact email | celine.leclercq@list.lu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD022712 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022712
- Label: PRIDE project
- Name: Integrative analysis of two russeted and non-russeted closely related apple varieties during fruit development provides new insights about the cuticle/suberin interplay