PXD022711-1

PXD022711 is an original dataset announced via ProteomeXchange.

Title	Proteome-wide analysis of heat-stress in Pinus radiata somatic embryos reveals a combined response of sugar metabolism and translational regulation mechanisms
Description	Recent research has demonstrated that high temperatures during initial stages of conifer somatic embryogenesis can modulate the subsequent phases of the process, as well as the behaviour of the resulting plants ex vitro. In this sense, the development of high-throughput techniques has facilitated the study of the molecular response of plants to numerous stress factors. Proteomics offers a reliable image of the cell status and is known to be extremely susceptible to environmental temperature changes. As a result, in this study, the proteome of radiata pine somatic embryos was studies by LC-MS after the application of high temperatures during induction of embryonal masses (23 °C, 8 weeks, control, 40 °C, 4 h, 60 °C, 5 min). Then, the content of specific soluble sugars and sugar alcohols by HPLC was analysed. Results confirmed a decreased initiation rate of embryonal masses under high temperatures and a slight increase in the production of somatic embryos, as observed in previous studies. Besides, heat provoked a long-term readjustment of the protein synthesis machinery, by regulating translation and the composition of ribosomes. The levels of proteins related to methylation and posttranscriptional regulation mechanisms were also altered under high temperatures, coupled with higher contents of heat shock proteins and chaperones, transmembrane transport proteins, enzymes involved in the synthesis of fatty acids, specific compatible sugars (myo-inositol), and changes in the composition of cell-wall carbohydrates. These modifications occurred at the expense of the synthesis of molecules involved in oxidative stress responses, such as peroxidases, thiamine, phenolic compounds and some amino acids, by a decreased activity of the glycolytic pathway and reduced nitrogen assimilation.
HostingRepository	PRIDE
AnnounceDate	2021-04-09
AnnouncementXML	Submission_2021-04-09_05:36:06.759.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Vera Mendes
SpeciesList	scientific name: Pinus radiata (Monterey pine); NCBI TaxID: 3347;
ModificationList	S-carboxamidoethyl-L-cysteine
Instrument	TripleTOF 6600

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-24 09:04:36	ID requested
⏵ 1	2021-04-09 05:36:07	announced

Dataset with its publication pending

submitter keyword: carbohydrates, compatible solutes, heat shock proteins, high temperatures, methylation, proteomics, radiata pine, somatic embryogenesis.

Paloma Moncaleán
contact affiliation	Department of Forestry Science, NEIKER, Arkaute, Spain.
contact email	pmoncalean@neiker.eus
lab head
Vera Mendes
contact affiliation	Center for Neuroscience and Cell Biology, University of Coimbra
contact email	vera3m@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022711
     1. Label: PRIDE project
     2. Name: Proteome-wide analysis of heat-stress in Pinus radiata somatic embryos reveals a combined response of sugar metabolism and translational regulation mechanisms