PXD022641 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | High fat diet mice intestinal epithelial exosomal proteins were analyzed by MSMS. |
Description | High-fat diet (HFD) decreases insulin sensitivity. How high-fat diet causes insulin resistance is largely unknown. Here, we show that lean mice become insulin resistant after being administered exosomes isolated from the feces of obese mice fed a high-fat diet (HFD) or from human type II diabetic patients with diabetes. HFD altered the lipid composition of exosomes from predominantly PE in exosomes from lean animals (L-Exo) to PC in exosomes from obese animals (H-Exo). Mechanistically, we show that intestinal H-Exo is taken up by macrophages and hepatocytes, leading to inhibition of the insulin signaling pathway. Moreover, exosome-derived PC binds to and activates AhR, leading to inhibition of the expression of genes essential for activation of the insulin signaling pathway, including IRS-2, and its downstream genes PI3K and Akt. Together, our results reveal HFD-induced exosomes as potential contributors to the development of insulin resistance. Intestinal exosomes thus have potential as broad therapeutic targets. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-23 |
AnnouncementXML | Submission_2021-03-23_12:02:21.111.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anil Kumar |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-20 02:57:54 | ID requested | |
⏵ 1 | 2021-03-23 12:02:23 | announced | |
Publication List
Kumar A, Sundaram K, Mu J, Dryden GW, Sriwastva MK, Lei C, Zhang L, Qiu X, Xu F, Yan J, Zhang X, Park JW, Merchant ML, Bohler HCL, Wang B, Zhang S, Qin C, Xu Z, Han X, McClain CJ, Teng Y, Zhang HG, High-fat diet-induced upregulation of exosomal phosphatidylcholine contributes to insulin resistance. Nat Commun, 12(1):213(2021) [pubmed] |
Keyword List
submitter keyword: L-Exo, H-Exo, Insulin resistance |
Contact List
Huang-Ge Zhang |
contact affiliation | Department of Microbiology & Immunology School of Medicine University of Louisville CTRB, Room 309 Louisville, KY, USA 40202 |
contact email | h0zhan17@louisville.edu |
lab head | |
Anil Kumar |
contact affiliation | University of Louisville |
contact email | a0kuma19@louisville.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022641
- Label: PRIDE project
- Name: High fat diet mice intestinal epithelial exosomal proteins were analyzed by MSMS.