PXD022637 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Sex and Insulin Resistance Define a Cell Autonomous Supernetwork of Protein Phosphorylation |
Description | Insulin resistance is an important factor in the pathophysiology of many metabolic disorders. The aim of this study was to uncover the cell autonomous determinants of insulin resistance using induced pluripotent stem cell (iPSC)-derived myoblasts (iMyos). Here, we found that iMyos from insulin resistant non-diabetic individuals show impaired insulin signaling, defective insulin-stimulated glucose uptake and glycogen synthase activity compared to insulin sensitive individuals. Global phosphoproteomic analysis uncovered a large network of altered protein phosphorylations in insulin resistance, mostly outside the canonical insulin-signaling cascade. More surprisingly, we observed striking differences in the phosphoproteomic signature from male versus female subjects, including changes in DNA and RNA processing, GTPase signaling, and SUMOylation/ubiquitination. These findings unravel cell autonomous mechanisms underlying insulin resistance and demonstrate a previously unrecognized supernetwork of cell signaling differences in males and females that must be considered in understanding the molecular basis of sex-based differences in normal physiology and disease. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-03 |
AnnouncementXML | Submission_2021-11-03_07:12:19.006.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Ashokkumar Jayavelu |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-19 06:28:35 | ID requested | |
⏵ 1 | 2021-11-03 07:12:19 | announced | |
Publication List
Haider N, Lebastchi J, Jayavelu AK, Batista TM, Pan H, Dreyfuss JM, Carcamo-Orive I, Knowles JW, Mann M, Kahn CR, Signaling defects associated with insulin resistance in nondiabetic and diabetic individuals and modification by sex. J Clin Invest, 131(21):(2021) [pubmed] |
Keyword List
submitter keyword: Sex, Insulin Resistance, Phosphorylation, Diabetes , Insulin Signaling |
Contact List
Prof.Matthias Mann |
contact affiliation | Director, Dept of Proteomics and Signal Transduction Max Planck Institute of Biochemistry |
contact email | mmann@biochem.mpg.de |
lab head | |
Ashokkumar Jayavelu |
contact affiliation | Dept Proteomics and Signal Transduction, MaxPlanck Institute of Biochemistry |
contact email | jayavelu@biochem.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD022637 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022637
- Label: PRIDE project
- Name: Sex and Insulin Resistance Define a Cell Autonomous Supernetwork of Protein Phosphorylation