PXD022623 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative proteomics of Histoplasma capsulatum |
Description | Histoplasma capsulatum is a thermally dimorphic fungus with worldwide distribution, and high incidence in the Americas. It is the etiologic agent of histoplasmosis, an important life-threatening systemic mycosis. Dimorphism is an important feature for fungal survival in different environments and it has been related to the virulence of H. capsulatum, and essential to the establishment of infection. Proteomic profiles have brought important contributions to the knowledge of metabolism and pathogenicity in several biological models. However, studies of the H. capsulatum proteome have been underexplored. In the present study, we report the first proteomic comparison between the mycelium and the yeast cells of H. capsulatum. Liquid chromatography coupled to mass spectrometry was used to evaluate the proteomic profile of the two phases of H. capsulatum. In summary, 214 proteins were only detected/or preferentially abundant in mycelium, while the same occurred to 335 proteins in yeast cells. In mycelium, enzymes related to the glycolytic pathway and to the alcoholic fermentation showed greater abundance, suggesting a higher use of anaerobic pathways for energy production. In yeast cells, proteins related to the tricarboxylic acid cycle and response to temperature stress showed high abundance. Proteins related to oxidative stress response or involved with cell wall metabolism were identified with differential abundance in both conditions. Validation of proteomic data was performed by enzymatic activity determination, western blot assays, or immunofluorescence microscopy. These experiments corroborated, directly or indirectly, the abundance of isocitrate lyase, 2-methylcitrate synthase, catalase B, and mannosyl-oligosaccharide-1,2-alpha-mannosidase in the mycelium and heat shock protein (HSP) 30, HSP60, glucosamine-fructose-6-phosphate aminotransferase, glucosamine-6-phosphate deaminase, and N-acetylglucosamine-phosphate mutase in yeast-cells. The proteomic profile associated functional classification analyzes of proteins provided a better understanding of the metabolic reorganization and cell wall remodeling on the yeast form of H. capsulatum. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-09 |
AnnouncementXML | Submission_2021-09-09_14:13:05.021.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | CLAYTON BORGES |
SpeciesList | scientific name: Histoplasma capsulatum; NCBI TaxID: 5037; |
ModificationList | phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-19 03:32:10 | ID requested | |
⏵ 1 | 2021-09-09 14:13:05 | announced | |
Publication List
Almeida MA, Baeza LC, Almeida-Paes R, Bail, ã, o AM, Borges CL, Guimar, ã, es AJ, Soares CMA, Zancop, é, -Oliveira RM, Yeast and Mycelium Reveals Differential Metabolic Shifts and Cell Wall Remodeling Processes in the Different Morphotypes. Front Microbiol, 12():640931(2021) [pubmed] |
Keyword List
submitter keyword: Histoplasma capsulatum, dimorphism, yeast, mycelium, proteomic analysis, fungal biology |
Contact List
CLAYTON LUIZ BORGES |
contact affiliation | Federal University of Goias |
contact email | clbluiz2@gmail.com |
lab head | |
CLAYTON BORGES |
contact affiliation | Biochemistry and molecular biology department, Universidade Federal de Goias, Brazil |
contact email | clbluiz2@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022623
- Label: PRIDE project
- Name: Comparative proteomics of Histoplasma capsulatum