PXD022607-2

PXD022607 is an original dataset announced via ProteomeXchange.

Title	The PI3Kδ interactome in naïve and activated mouse CD4+ T cells
Description	Phosphoinositide 3-kinases (PI3Ks) play a central role in adaptive immunity by transducing signals from the T cell antigen receptor (TCR) via production of PIP3. PI3Kδ is a heterodimer composed of a p110δ catalytic subunit associated with a p85α or p85β regulatory subunit and is preferentially engaged by the TCR upon T cell activation. The molecular mechanisms leading to PI3Kδ recruitment and activation at the TCR signalosome remain unclear. In this study, we have used affinity purification coupled with quantitative mass spectrometry to uncover the p110δ interactome in primary CD4+ T cells. Moreover, we have determined how the PI3Kδ interactome changes upon the differentiation of small naïve T cells into T cell blasts expanded in the presence of IL-2.
HostingRepository	PRIDE
AnnounceDate	2021-03-08
AnnouncementXML	Submission_2021-12-07_03:40:48.537.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Daisy Luff
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated N-terminal; monohydroxylated residue; iodoacetamide derivatized residue; TMT6plex-126 reporter+balance reagent N6-acylated lysine
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-18 08:03:58	ID requested
1	2021-03-07 22:32:49	announced
⏵ 2	2021-12-07 03:40:49	announced	2021-12-07: Updated publication reference for PubMed record(s): 33763075.

Luff DH, Wojdyla K, Oxley D, Chessa T, Hudson K, Hawkins PT, Stephens LR, Barry ST, Okkenhaug K, T Cells. Front Immunol, 12():631271(2021) [pubmed]

submitter keyword: PI3K, PI3Kδ, T-cells, TCR, CD4+, interactome, AP-MS, nLC-MS/MS, TMT, 10-plex, mouse

Klaus Okkenhaug
contact affiliation	Department of Pathology, University of Cambridge, Cambridge, UK
contact email	ko256@cam.ac.uk
lab head
Daisy Luff
contact affiliation	The Francis Crick Institute, London, UK
contact email	daisy.luff@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD022607

PRIDE project URI

• PRIDE
  1. PXD022607
     1. Label: PRIDE project
     2. Name: The PI3Kδ interactome in naïve and activated mouse CD4+ T cells