PXD022585-2

PXD022585 is an original dataset announced via ProteomeXchange.

Title	An Isocaloric High-Fat Diet Improves Lifespan in Rats and Drosophila
Description	The health effect of dietary fat has been one of the most vexing issues in the nutrition field. Few animal studies have examined the impact of high-fat diets on lifespan by controlling energy intake. In this study, we found that compared to a normal diet, an isocaloric high-fat diet (IHF) significantly prolonged lifespan by decreasing the profiles of free fatty acids (FFAs) in serum and multiple tissues by downregulating FFAs anabolism and upregulating catabolism pathways in rats and flies. Proteomics analysis in rats identified PPRC1 as a key protein that was significantly upregulated by nearly 2-fold by IHF, and among the FFAs, only palmitic acid (PA) was robustly and negatively associated with the expression of PPRC1. Using PPRC1 transgenic RNAi/overexpression flies and in vitro experiments, we further demonstrated that IHF significantly reduced PA, which could upregulate PPRC1 through PPARG, resulting in improvements in oxidative stress and inflammation and prolonging lifespan.
HostingRepository	PRIDE
AnnounceDate	2021-01-06
AnnouncementXML	Submission_2021-01-17_23:19:11.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	dan shi
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	iTRAQ8plex-116 reporter+balance reagent acylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-17 06:09:36	ID requested
1	2021-01-05 23:45:19	announced
⏵ 2	2021-01-17 23:19:11	announced	2021-01-18: Updated publication reference for PubMed record(s): 33440166.

Shi D, Han T, Chu X, Lu H, Yang X, Zi T, Zhao Y, Wang X, Liu Z, Ruan J, Liu X, Ning H, Wang M, Tian Z, Wei W, Sun Y, Li Y, Guo R, Wang Y, Ling F, Guan Y, Shen D, Niu Y, Li Y, Sun C, An isocaloric moderately high-fat diet extends lifespan in male rats and Drosophila. Cell Metab, 33(3):581-597.e9(2021) [pubmed]

submitter keyword: Isocaloric, High-Fat Diet, Lifespan, Proteomics

Chang Hao Sun
contact affiliation	National Key Discipline Laboratory, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin, P. R. China
contact email	changhaosun2002@163.com
lab head
dan shi
contact affiliation	chongqing medical university
contact email	shidand2018@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/01/PXD022585

PRIDE project URI

• PRIDE
  1. PXD022585
     1. Label: PRIDE project
     2. Name: An Isocaloric High-Fat Diet Improves Lifespan in Rats and Drosophila