PXD022565 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Transfection with Glucuronidase A affects general proteome pattern in Giardia lamblia trophozoites |
Description | Giardia lamblia is an important causative agent of persistent diarrhea in humans, domestic animals, and cattle. Basic research is usually performed with the strain WBC6 and includes genetic manipulations such as transfections. Here, we investigate how transfection with a plasmid causing stable expression of a foreign gene affects the whole proteome pattern. Using shotgun mass spectrometry, we compare the proteomes of untransfected trophozoites to trophozoites transfected with Escherichia coli glucuronidase A (GusA). Besides GusA, which is detected in the transfected trophozoites only, the proteomes of untransfected and transfected trophozoites differ by 132 differentially expressed proteins. In particular, transfection induces antigenic variation. Since transfection causing stable expression affects the proteome pattern, transfection experiments should take into account this effect. Due to a unique peptide panel, GusA is an example for a suitable internal standard for experiments involving transfected cells. |
HostingRepository | PRIDE |
AnnounceDate | 2021-03-04 |
AnnouncementXML | Submission_2021-03-04_14:44:02.327.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Manfred Heller |
SpeciesList | scientific name: Giardia intestinalis assemblage B; NCBI TaxID: 1394984; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2020-11-16 08:01:39 | ID requested | |
⏵ 1 | 2021-03-04 14:44:03 | announced | |
Publication List
Heller M, Braga S, M, ü, ller N, M, ü, ller J, Trophozoites. Front Cell Infect Microbiol, 10():602756(2020) [pubmed] |
Keyword List
submitter keyword: controls and standards in proteomic experimental procedures |
model organisms |
reverse genetics |
untargeted proteomics |
Contact List
Norbert Müller |
contact affiliation | Institute of parasitology Vetsuisse University of Bern |
contact email | norbert.mueller@vetsuisse.unibe.ch |
lab head | |
Manfred Heller |
contact affiliation | Proteomics and Mass Spectrometry Core Facility, Department for BioMedical Research (DBMR), University of Bern, Bern, Switzerland |
contact email | pmscf@dbmr.unibe.ch |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD022565
- Label: PRIDE project
- Name: Transfection with Glucuronidase A affects general proteome pattern in Giardia lamblia trophozoites