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PXD022562-1

PXD022562 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInteraction of Chinese cabbage (Brassica rapa ssp. pekinensis) cell wall proteins with mustard leaf beetle (Phaedon cochleariae) polygalacturonase family members
DescriptionPlants possess various defense strategies to counter attacks from microorganisms or herbivores. For example, plants reduce the cell-wall-macerating activity of pathogen- or insect-derived polygalacturonases (PGs) by expressing PG-inhibiting proteins (PGIPs). PGs and PGIPs belong to multi-gene families believed to have been shaped by an evolutionary arms race. The mustard leaf beetle Phaedon cochleariae expresses both active PGs and catalytically inactive PG pseudoenzymes. Previous studies demonstrated that (i) PGIPs target beetle PGs and (ii) the role of PG pseudoenzymes remains elusive, despite having been linked to the pectin degradation pathway. For further insight into the interaction between plant PGIPs and beetle PG family members, we combined affinity purification with proteomics and gene expression analyses, and identified novel inhibitors of beetle PGs from Chinese cabbage (Brassica rapa ssp. pekinensis). A beetle PG pseudoenzyme was not targeted by PGIPs, but instead interacted with PGIP-like proteins. Phylogenetic analysis revealed that PGIP-like proteins clustered apart from classical PGIPs but together with proteins, which have been involved in developmental processes. Our results indicate that PGIP-like proteins represent not only interesting novel PG inhibitor candidates in addition to classical PGIPs, but also fascinating new players in the arms race between herbivorous beetles and plant defenses.
HostingRepositoryPRIDE
AnnounceDate2021-06-07
AnnouncementXMLSubmission_2021-06-06_22:39:42.398.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD022562
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterWiebke Haeger
SpeciesList scientific name: Phaedon cochleariae; NCBI TaxID: 80249; scientific name: Brassica rapa subsp. pekinensis; NCBI TaxID: 51351;
ModificationListiodoacetamide derivatized residue
InstrumentQ-Tof Ultima
Dataset History
RevisionDatetimeStatusChangeLog Entry
02020-11-16 07:17:24ID requested
12021-06-06 22:39:42announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: beetle, polygalacturonase, polygalacturonase-inhibiting proteins, cell wall proteins, Chinese cabbage, cell wall-degrading enzymes, LC-MS/MS, pull-down
Contact List
Yannick Pauchet
contact affiliationMax Planck Institut for Chemical Ecology, Department of Entomology, Project Group Molecular Biology of the Insect Digestive System, Germany
contact emailypauchet@ice.mpg.de
lab head
Wiebke Haeger
contact affiliationMax Planck Institute for Chemical Ecology
contact emailwihaeger@ice.mpg.de
dataset submitter
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Dataset FTP location
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