PXD022557-1

PXD022557 is an original dataset announced via ProteomeXchange.

Title	Sex Impacts Cardiac Function and the Proteome Response to Thyroid Hormone in Aged Mice
Description	Background: Sex and age have substantial influence on thyroid function. Sex influences the risk and clinical expression of thyroid disorders (TDs), with age a proposed trigger for the development of TDs. Cardiac function is affected by thyroid hormone levels with gender differences. Accordingly, we investigated the proteomic changes involved in sex based cardiac responses to thyroid dysfunction in elderly mice. Methods: Aged (18-20 months) male and female C57BL/6 mice were fed diets to create euthyroid, hypothyroid, or hyperthyroid states. Serial echocardiographs were performed to assess heart function. Proteomic changes in cardiac protein profiles were assessed by 2-D DIGE and LC-MS/MS, and a subset confirmed by immunoblotting. Results: Serial echocardiographs showed ventricular function remained unchanged regardless of treatment. Heart rate and size increased (hyperthyroid) or decreased (hypothyroid) independent of sex. Pairwise comparison between the six groups identified 55 proteins (≥ 1.5-fold difference and p < 0.1). Compared to same-sex controls 26/55 protein changes were in the female hypothyroid heart, whereas 15/55 protein changes were identified in the male hypothyroid, and male and female hyperthyroid heart. The proteins mapped to oxidative phosphorylation, tissue remodeling and inflammatory response pathways. Conclusion: We identified both predicted and novel proteins with gender specific differential expression in response to thyroid hormone status, providing a catalogue of proteins associated with thyroid dysfunction. Pursuit of these proteins and their involvement in cardiac function will expand our understanding of mechanisms involved in sex-based cardiac response to thyroid dysfunction.
HostingRepository	PRIDE
AnnounceDate	2020-12-23
AnnouncementXML	Submission_2020-12-22_16:31:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dolena Ledee
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	5800 TOF/TOF

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-16 03:39:44	ID requested
⏵ 1	2020-12-22 16:31:25	announced

Dataset with its publication pending

submitter keyword: Thyroid hormone, heart, aged, LC-MS/MS

Dolena Ledee
contact affiliation	1. Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, WA 2. Division of Cardiology, Department od=f Pediatrics, University of Washington, Seattle, WA
contact email	dolena.ledee@seattlechildrens.org
lab head
Dolena Ledee
contact affiliation	Seattle Children's Research Institute
contact email	dolena.ledee@seattlechildrens.org
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD022557
     1. Label: PRIDE project
     2. Name: Sex Impacts Cardiac Function and the Proteome Response to Thyroid Hormone in Aged Mice