PXD022539-1

PXD022539 is an original dataset announced via ProteomeXchange.

Title	Cold-induced phosphorylational sites analysis of protein kinase OST1
Description	For the analysis of phosphorylation sites of OST1, 8 g pSuper:OST1-Myc plants materials, treated with 4oC for 0 or 2 h, were used for protein extraction and immunoprecipitated with anti-Myc agarose. Then OST1-Myc proteins were eluted with 0.1 M glycine solution (pH 2.8) and mixed with 1 M Tris solution to ensure the pH of OST1-Myc is 7.5. After the concentration of PP2CG1 proteins with 30K-ultrafiltration tubes, phosphatase assay was performed: OST1-Myc was treated with GST-PP2CG1 for 0 or 60 min under 30oC. Then, the samples were digested with trypsin using FASP method. The collected solution containing digested peptide were lyophilized for phospho-peptides enrichment, which was performed using TiO2-tips (Thermo Scientific, #A32992) according to the manufacture’s protocol. The phosphor-peptides were resuspended in 0.1% FA for nano LC-MS analysis.
HostingRepository	iProX
AnnounceDate	2020-11-16
AnnouncementXML	Submission_2021-04-20_01:22:52.047.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jian Lv
SpeciesList	scientific name: Arabidopsis thaliana; NCBI TaxID: 3702;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2020-11-15 19:31:59	ID requested
⏵ 1	2021-04-20 01:22:52	announced

Lv J, Liu J, Ming Y, Shi Y, Song C, Gong Z, Yang S, Ding Y, Reciprocal regulation between the negative regulator PP2CG1 phosphatase and the positive regulator OST1 kinase confers cold response in Arabidopsis. J Integr Plant Biol, 63(8):1568-1587(2021) [pubmed]

submitter keyword: phospho-sites, OST1, Cold-induced

Shuhua Yang
contact affiliation	China Agricultural University
contact email	yangshuhua@cau.edu.cn
lab head
Jian Lv
contact affiliation	China Agricultural University
contact email	734999889@qq.com
dataset submitter

iProX dataset URI